Zymogenic nucleic acid molecules

• US 6,201,113 B1
• Filed: 08/11/2000
• Issued: 03/13/2001
• Est. Priority Date: 03/05/1998
• Status: Expired due to Term

• Current Assignee: Alison V. Todd, Caroline J. Fuery, Murray J. Cairns
• Original Assignee: Alison V. Todd, Caroline J. Fuery, Murray J. Cairns
• Inventors: Todd, Alison V., Fuery, Caroline J., Cairns, Murray J.
• Primary Examiner(s): Horlick, Kenneth R.
• Application Number:
  US09/637,405
• Time in Patent Office: 214 Days
• Field of Search: 536/23.2, 536/24.33
• US Class Current: 536/23.2
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6823 Release of bound markers
• CPC Class Codes: C12Q 1/6844 Nucleic acid amplification reactions
• CPC Class Codes: C12Q 1/6853 using modified primers or templates
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 1/6867 Replicase-based amplification, e.g. using Q-beta replicase
• CPC Class Codes: C12Q 2521/337 Ribozyme
• CPC Class Codes: C12Q 2525/149 incorporating a coding sequence
• CPC Class Codes: C12Q 2525/161 incorporating target specific and non-target specific sites
• CPC Class Codes: C12Q 2531/113 PCR
• CPC Class Codes: C12Q 2531/125 Rolling circle
• CPC Class Codes: C12Q 2537/143 Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
• CPC Class Codes: C12Q 2545/114 involving a quantitation step
• CPC Class Codes: C12Q 2563/125 the label being enzymatic, i.e. proteins, and non proteins, such as nucleic acid with enzymatic activity

Abstract

This application provides methods of detecting and quantitatively determining a target nucleic acid sequence in a sample, which comprise contacting the sample with a primer and a zymogene which encodes, but which itself is the anti-sense sequence of, a catalytic nucleic acid sequence, so that when the target is present, a single amplified nucleic acid molecule is produced which comprises the sequences of both the target and catalytic molecules. This invention further provides a method of simultaneously detecting the presence of a plurality of target nucleic acid sequences in a sample. Finally, this invention provides molecules and kits for practicing the instant methods.

Claims

1. A DNA molecule comprising a primer and a DNAzyme-encoding zymogene, wherein the primer is situated 3′

of the zymogene.