Production of recombinant proteins in semen
First Claim
1. A method for the production of a heterologous protein in semen of a mouse comprising the steps of:
- production a transgenic mouse whose genome comprises an expression system comprising in operable association a p12 promoter, a DNA sequence encoding a signal peptide and a DNA sequence encoding a protein heterologous to the promoter, wherein expression of said DNA sequences results in the production and secretion of said protein of interest into the mouse'"'"'s semen to a detectable level; and
collecting semen produced by said transgenic mouse.
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Abstract
The present invention relates to a method for the production and secretion into animal'"'"'s semen of an exogenous recombinant protein comprising the steps of: a) producing a non-human transgenic animal characterized by an expression system comprising a promoter specific for the genital tract or accessory glands operatively linked to an exogenous DNA sequence coding for the recombinant protein through a DNA sequence coding for a signal peptide effective in secreting and maturing the recombinant protein in genital tract tissue; b) collecting semen produced by the non-human transgenic animal; and c) isolating the exogenous recombinant protein from the semen.
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Citations
6 Claims
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1. A method for the production of a heterologous protein in semen of a mouse comprising the steps of:
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production a transgenic mouse whose genome comprises an expression system comprising in operable association a p12 promoter, a DNA sequence encoding a signal peptide and a DNA sequence encoding a protein heterologous to the promoter, wherein expression of said DNA sequences results in the production and secretion of said protein of interest into the mouse'"'"'s semen to a detectable level; and
collecting semen produced by said transgenic mouse. - View Dependent Claims (2, 3, 4, 5)
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6. A transgenic mouse whose genome comprises in operable association a p12 promoter, a DNA sequence encoding a signal peptide and a DNA sequence encoding a protein heterologous to the promoter, wherein expression of said DNA sequences results in the production and secretion of said protein of interest into the mouse'"'"'s semen to a detectable level.
Specification