Tissue factor based prothrombin time reagent

• US 6,203,816 B1
• Filed: 04/24/1995
• Issued: 03/20/2001
• Est. Priority Date: 11/13/1990
• Status: Expired due to Term

• Current Assignee: Corvas International, Inc. (Bausch Health Cos., Inc.)
• Original Assignee: Corvas International, Inc. (Bausch Health Cos., Inc.)
• Inventors: Brown, Scott M.
• Primary Examiner(s): Kishore, Gollamudi S.
• Application Number:
  US08/427,683
• Time in Patent Office: 2,157 Days
• Field of Search: 424/450, 424/812, 264/4.1, 264/4.3, 436/69, 436/829, 514/21
• US Class Current: 424/450
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: G01N 33/86 involving blood coagulating time or factors, or their receptors

Abstract

The present disclosure relates to novel liposome compositions in which tissue factor is incorporated and methods for their preparation. The composition of the phospholipids in the liposomes has been adjusted to allow maximum coagulant activity and sensitivity to extrinsic coagulation factors of the incorporated tissue factor.

Claims

1. A method for incorporating active substantially purified natural or recombinant tissue factor into a lipid bilayer of phospholipid liposomes comprising the steps of:

(a) co-solubilizing purified phospholipids, substantially purified tissue factor, and carrier proteins with detergent and cryopreservative agent and thereby causing formation of mixed micelles of phospholipids, tissue factor, carrier proteins, detergent and cryopreservative agent;

(b) removing detergent and thereby causing the micelles to form liposomes; and

(c) adding cadmium salt.

2. The method according to claim 1, wherein the phospholipids are phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine.

3. The method according to claim 1, wherein the detergent is selected form the group consisting of CHAPS, octyl beta-D-glucopyranoside, and octyl beta-D-thioglucopyranoside.

4. The method according to claim 1, wherein the detergent is removed by the technique selected from the group consisting of dialysis, tangential flow diafiltration, and chromatographic means.

5. A method for incorporating substantially purified active natural or recombinant tissue into phospholipid micelles comprising the steps of:

(a) co-solubilizing purified phospholipids, substantially purified tissue factor, and carrier proteins with detergent and thereby causing formation of mixed micelles of phospholipids, tissue factor, carrier proteins, and detergent; and

(b) adding cadmium salt to said micelles, wherein the detergent is not removed from the mixed micelles.

6. The method according to claim 5, wherein the phospholipids are phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine.

7. The method according to claim 5, wherein the detergent is octyl beta-D-glucopyranoside or octyl beta-D-thioglucopyranoside.