Metallic overcoating as a light attenuating layer for optical sensors

• US 6,207,110 B1
• Filed: 08/21/1998
• Issued: 03/27/2001
• Est. Priority Date: 08/21/1998
• Status: Expired due to Term

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: Bayer Corporation (Bayer AG)
• Inventors: Sullivan, Kevin J., Collins, Thomas C., Slovacek, Rudolf E.
• Primary Examiner(s): SNAY, JEFFREY R
• Application Number:
  US09/137,728
• Time in Patent Office: 949 Days
• Field of Search: 422/82.05, 422/82.06, 422/82.07, 422/82.08, 422/82.11, 422/56, 422/57
• US Class Current: 422/429
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53

Abstract

A liquid permeable metallic coating is utilized in conjunction with a fluorescence based optical sensor. The metallic coating is deposited directly on, and is in physical contact with, the sensing membrane. The metallic coating does not require an intervening substrate layer or other components. When light from a light source is shone through the substantially light transmissive substrate onto the sensing membrane, the metallic overcoating reflects back the excitation light as well as the fluorescence light generated by the sensor such that substantially no light reaches the sample where the light may be scattered and/or absorbed by the sample. Accordingly, the accuracy and repeatability of the sensor is improved while the cost and production times associated with manufacturing the sensor are minimized.

Claims

1. A sensor comprising:

a substrate substantially transmissive to light having a first side and a second side;

a sensing membrane layered onto the second side of said substrate; and

a liquid permeable, substantially nontransmissive to light metallic coating comprising gold and palladium directly layered on said sensing membrane.

2. The sensor of claim 1 wherein said metallic coating is sputter coated onto said sensing membrane.

3. The sensor of claim 1 wherein said metallic coating is evaporated onto said sensing membrane.

4. The sensor of claim 1 wherein said metallic coating has an optical density of between approximately 0.2 and approximately 0.893.

5. The sensor of claim 1 wherein said sensing membrane comprises a mixture of ethylhexylmethacrylate, methylmethacrylate and an oxygen sensing dye.

6. The sensor of claim 5 wherein said oxygen sensing dye comprises octa-ethyl-Pt-porphyrin (OEP).

7. The sensor of claim 1 wherein said substrate comprises glass.

8. The sensor of claim 1 wherein said substrate is selected from the group consisting of cellulose acetate, cellulose acetate butyrate, polyethylene terephthalate, bisphenol A polycarbonate, polystyrene, and polymethyl methacrylate.

9. The sensor of claim 1 further comprising:

a light source in communication with said substrate, said sensing membrane and said metallic coating; and

a detector in communication with said substrate, said sensing membrane and said metallic coating.

10. A sensor comprising:

a substrate substantially transmissive to light having a first side and a second side;

a sensing membrane layered onto the second side of said substrate; and

a liquid permeable, substantially nontransmissive to light metallic coating comprising gold and palladium layered directly on said sensing membrane, said metallic coating having an optical density of approximately 0.893;

a light source in communication with said substrate, said sensing membrane, and said metallic coating; and

a detector in communication with said substrate, said sensing membrane and said metallic coating.