Fluorescent imaging method of saccharide uptake activity of living tissue

• US 6,207,136 B1
• Filed: 01/15/1998
• Issued: 03/27/2001
• Est. Priority Date: 03/11/1997
• Status: Expired due to Fees

• Current Assignee: Tokyo University of Agriculture and Technology
• Original Assignee: Tokyo University of Agriculture and Technology
• Inventors: Matsuoka, Hideaki
• Primary Examiner(s): JONES, DAMERON LEVEST
• Application Number:
  US09/007,410
• Time in Patent Office: 1,167 Days
• Field of Search: 424/1.11, 424/1.65, 424/1.73, 424/9.1, 424/9.6, 424/9.7, 424/9.8, 424/9.81, 127/30, 127/36, 514/23, 436/95, 536/1.11, 422/82.08, 252/301.16, 250/458.1, 206/569, 206/570
• US Class Current: 424/9.6
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: C07H 15/26 Acyclic or carbocyclic radicals, substituted by hetero rings
• CPC Class Codes: C07H 5/06 Aminosugars

Abstract

A fluorescent imaging method of the changes in viability of living bodies wherein the living bodies are provided with a chemical or physical exterior stimulation and information on the changes in viability of cells caused by the stimulation is obtained by means of a fluorescent imaging method of the saccharide uptake activity by contacting or administering to the living bodies an aqueous solution of a fluorescent saccharide compound which includes at its specific position a saccharide molecule chemically combined with a fluorescent pigment molecule.

Claims

1. A method of detecting the saccharide uptake activity of living tissue containing living bodies having biological activity comprising the steps of:

forming an aqueous solution of a fluorescent saccharide compound comprising a saccharide molecule chemically combined at a specific position thereof with a fluorescent pigment molecule having a molecular weight no greater than 300;

contacting said tissue with said aqueous solution of the fluorescent saccharide compound such that said fluorescent saccharide compound is incorporated therein; and

analyzing the tissue having the fluorescent saccharide compound incorporated therein with a fluorescence detection device.

2. The method of claim 1, wherein said saccharide is selected from the group consisting of galactose, mannose and N-acetylglucosamine.

3. The method of claim 1, additionally comprising the step of chemically or physically stimulating the exterior of the tissue prior to analyzing the tissue with the fluorescence detection device.

4. The method according to claim 1, wherein said saccharide molecule is glucose, and said specific position is the C-2 position in the glucose.

5. The method according to claim 1, wherein said fluorescent saccharide compound is 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose.

6. The method according to claim 1, wherein said fluorescent saccharide compound is contained at a concentration of 10 μ

M to 10 mM in the aqueous solution.

7. The method according to claim 3, wherein said exterior stimulation is any one of chemical stimulation with medicaments, poisons, microorganisms, and physical stimulation with light, heat, pressure changes, sonic waves, electric fields, magnetic fields, electromagnetic fields and radioactivity.