Fluorescent dyes (AIDA) for solid phase and solution phase screening
First Claim
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1. Compounds represented by formula (I) whereinone of the radicals R1 or R2 and one of the radicals R3 or R4 is hydrogen and the other is independently —
- COOH, —
COOR7, —
CONH2, —
CONH(CH2),OH, wherein n=2-8, —
CONR8R9, —
CH2OH, —
CH2NH2, —
NO2, NR10OR11, NHCOR12, Cl, Br, F, —
CF3, —
N=C=O, N=C=S, —
SO3H, —
SO2NH(CH2)nNH2, (C1-C4) alkyl, (C1-C16)-alkyl substituted at the terminal carbon with —
COOH, —
COOR7, —
CONH2, —
CONR8R9, —
CONH(CH2)nOH, wherein n=2-8, —
CH2OH, —
CH2NH2, —
N=C=O, N=C=S, —
SO3H, —
SO2NH(CH2)nNH2, —
CONH(CH2)nNH2 wherein n=2-8, and the NH2-group could also be substituted by (C1-C4) alkyl or a commonly used amino protecting group, and one of the radicals R5 or R6 is hydrogen and the other is hydrogen, halogen, —
NO2, NR10OR11, NHCOR12, (C1-C4) alkyl, (C1-C16)-alkyl substituted at the terminal carbon with —
COOH, —
COOR7, —
CONH2, —
CONR8R9, —
CONH(CH2)nOH, wherein n=2-8, —
CH2OH, —
CH2NH2 , —
N=C=O, N=C=S, —
SO3H, —
SO2NH(CH2)nNH2, —
CONH(CH2)nNH2 wherein n=2-8, and the NH2-group could also be substituted by (C1-C4) alkyl or a commonly used amino protecting group, with the proviso that only one of R1-R6 is nitro, R7 is a commonly used carboxyl protecting or carboxyl activating group, R8 or R9 is hydrogen and the other is lower alkyl (C1-C4), phenyl, benzyl, or R8 and R9 are part of a 5 or 6 membered ring, R10 and R11 are independently hydrogen or (C1-C4)alkyl, and R12 is (C1-C10)alkyl or phenyl, which both can be substituted by (C1-C4) alkyl, protected amino group or halogen.
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Abstract
The invention relates to new fluorescent dyes of formula (I)
which can be used in high throughput screening both, on the solid phase as well as in homogeneous solution.
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Citations
3 Claims
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1. Compounds represented by formula (I)
wherein one of the radicals R1 or R2 and one of the radicals R3 or R4 is hydrogen and the other is independently — - COOH, —
COOR7, —
CONH2, —
CONH(CH2),OH, wherein n=2-8, —
CONR8R9, —
CH2OH, —
CH2NH2, —
NO2, NR10OR11, NHCOR12, Cl, Br, F, —
CF3, —
N=C=O, N=C=S, —
SO3H, —
SO2NH(CH2)nNH2, (C1-C4) alkyl, (C1-C16)-alkyl substituted at the terminal carbon with —
COOH, —
COOR7, —
CONH2, —
CONR8R9, —
CONH(CH2)nOH, wherein n=2-8, —
CH2OH, —
CH2NH2, —
N=C=O, N=C=S, —
SO3H, —
SO2NH(CH2)nNH2, —
CONH(CH2)nNH2 wherein n=2-8, and the NH2-group could also be substituted by (C1-C4) alkyl or a commonly used amino protecting group,and one of the radicals R5 or R6 is hydrogen and the other is hydrogen, halogen, —
NO2, NR10OR11, NHCOR12, (C1-C4) alkyl, (C1-C16)-alkyl substituted at the terminal carbon with —
COOH, —
COOR7, —
CONH2, —
CONR8R9, —
CONH(CH2)nOH, wherein n=2-8, —
CH2OH, —
CH2NH2 , —
N=C=O, N=C=S, —
SO3H, —
SO2NH(CH2)nNH2, —
CONH(CH2)nNH2 wherein n=2-8, and the NH2-group could also be substituted by (C1-C4) alkyl or a commonly used amino protecting group,with the proviso that only one of R1-R6 is nitro, R7 is a commonly used carboxyl protecting or carboxyl activating group, R8 or R9 is hydrogen and the other is lower alkyl (C1-C4), phenyl, benzyl, or R8 and R9 are part of a 5 or 6 membered ring, R10 and R11 are independently hydrogen or (C1-C4)alkyl, and R12 is (C1-C10)alkyl or phenyl, which both can be substituted by (C1-C4) alkyl, protected amino group or halogen. - View Dependent Claims (2, 3)
- COOH, —
Specification