Calcium phosphate microcarriers and microspheres

• US 6,210,715 B1
• Filed: 02/02/2000
• Issued: 04/03/2001
• Est. Priority Date: 04/01/1997
• Status: Expired due to Term

• Current Assignee: Cap Biotechnology Inc.
• Original Assignee: Cap Biotechnology Inc.
• Inventors: Starling, L. Brian, Stephan, James E.
• Primary Examiner(s): Page, Thurman K.
• Assistant Examiner(s): FUBARA, BLESSING M
• Application Number:
  US09/402,308
• Time in Patent Office: 426 Days
• Field of Search: 604/93, 623/11, 210/502.1, 424/489, 424/423, 424/426, 424/490, 424/602
• US Class Current: 424/489
• CPC Class Codes: A61F 2/30767 Special external or bone-contacting surface, e.g. coating for improving bone ingrowth
• CPC Class Codes: A61F 2002/2817 Bone stimulation by chemical reactions or by osteogenic or biological products for enhancing ossification, e.g. by bone morphogenetic or morphogenic proteins [BMP] or by transforming growth factors [TGF]
• CPC Class Codes: A61F 2002/30242 spherical
• CPC Class Codes: A61F 2002/30593 hollow
• CPC Class Codes: A61F 2230/0071 spherical
• CPC Class Codes: A61F 2310/00293 containing a phosphorus-containing compound, e.g. apatite
• CPC Class Codes: A61F 2310/00796 Coating or prosthesis-covering structure made of a phosphorus-containing compound, e.g. hydroxy(l)apatite
• CPC Class Codes: A61K 9/1611 Inorganic compounds
• CPC Class Codes: A61K 9/501 Inorganic compounds
• CPC Class Codes: A61L 27/12 Phosphorus-containing materials, e.g. apatite
• CPC Class Codes: A61L 31/026 Ceramic or ceramic-like structures, e.g. glasses
• CPC Class Codes: B01J 20/048 containing phosphorus, e.g. phosphates, apatites, hydroxyapatites
• CPC Class Codes: B01J 20/28004 Sorbent size or size distribution, e.g. particle size
• CPC Class Codes: B01J 20/28011 Other properties, e.g. density, crush strength
• CPC Class Codes: B01J 20/28019 Spherical, ellipsoidal or cylindrical
• CPC Class Codes: B01J 20/28021 Hollow particles, e.g. hollow spheres, microspheres or cenospheres
• CPC Class Codes: B01J 20/2803 Sorbents comprising a binder, e.g. for forming aggregated, agglomerated or granulated products
• CPC Class Codes: B01J 20/282 Porous sorbents ion exchange B01J39/00 - B01J41/00
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography
• CPC Class Codes: C01B 25/32 Phosphates of magnesium, calcium, strontium, or barium

Abstract

The present invention provides calcium phosphate-based (CAP) microcarriers and microspheres and their use in cell culturing systems, chromatography and implantable biomedical materials.

Claims

1. A hollow calcium phosphate (CaP) microbead having a density from about 1.00 grams/cc to about 2.00 grams/cc.

2. A hollow microbead according to claim 1, wherein the CaP microbead comprises hydroxylapatite (HA), tricalcium phosphate (TCP), other calcium phosphate material, or a combination of at least two of HA, TCP or other calcium phosphate material.

3. A hollow microbead according to claim 2 comprising hydroxylapatite (HA), tricalcium phosphate (TCP), or a combination thereof.

4. A hollow microbead according to claim 1 comprising a wall, wherein the wall is essentially impermeable to aqueous media.

5. A hollow microbead according to claim 1 having an average diameter in an essentially spherical shape of from about 100 micrometers to about 6 millimeters.

6. A hollow microbead according to claim 1 further comprising a porous coating.

7. A hollow microbead according to claim 1 further comprising a biological coating.

8. A hollow microbead according to claim 7 wherein the biological coating is a growth factor.

9. A hollow CaP microbead of claim 1 having a density from about 1.2 grams/cc to about 2.0 grams/cc.

10. A biomedical implant comprising a microbead according to claim 1.

11. A chromatographic column comprising a hollow microbead according to claim 1.

12. An aggregate comprising the hollow microbead of claim 1.

13. A method of augmenting tissue comprising implanting the biomedical implant of claim 10.

14. A hollow CaP microbead according to claim 1, wherein said microbead has a density of about 1.01 grams/cc to about 1.12 grams/cc.