Transgenically produced prolactin

• US 6,210,736 B1
• Filed: 06/15/1998
• Issued: 04/03/2001
• Est. Priority Date: 06/17/1997
• Status: Expired due to Fees

• Current Assignee: GTC Biotherapeutics, Inc.
• Original Assignee: Genzyme Transgenics Corporation
• Inventors: Echelard, Yann, Wilburn, Brian
• Primary Examiner(s): Hauda, Karen M.
• Assistant Examiner(s): Shukla, Ram R.
• Application Number:
  US09/094,781
• Time in Patent Office: 1,023 Days
• Field of Search: 800/4, 530/412, 530/399, 530/397, 424/157.2, 424/157.1, 514/2, 426/580
• US Class Current: 426/580
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A23C 2230/05 Milk or milk products from transgenic animals
• CPC Class Codes: C07K 14/57554 Prolactin

Abstract

Transgenically produced prolactin and methods of making and using transgenically produced prolactin are disclosed.

Claims

1. A milk preparation obtained from a transgenic mammal whose genome contains a transgene wherein said transgene comprises a polynucleotide encoding a prolactin protein operably linked to a mammary gland specific promoter, wherein said prolactin protein is expressed in the mammary epithelial cells of said transgenic mammal and wherein less than 20% of the prolactin molecules in the milk preparation of the transgenic mammal are glycosylated.

2. The milk preparation of claim 1, wherein less than 10% of the prolactin molecules in the milk preparation of the transgenic mammal are glycosylated.

3. The milk preparation of claim 1, wherein less than 5% of the prolactin molecules in the milk preparation of the transgenic mammal are glycosylated.

4. The milk preparation of claim 1, wherein the prolactin is human prolactin.

5. The milk preparation of claim 1, wherein the transgenic mammal is a goat.

6. The milk preparation of claim 1, wherein the milk preparation comprises at least 1 mg/ml prolactin.