Array-based detection of genetic alterations associated with disease
First Claim
Patent Images
1. A method of screening for the presence of breast cancer cells in a sample, the method comprising:
- contacting a nucleic acid sample from a human patient with a probe which binds selectively to a target polynucleotide sequence on a region in which copy number is altered in breast cancer cells and is selected from the group consisting of Flpter 0.603, 0.646, 0.675 0.694, 0.772 or 0.867 on chromosome 20, wherein the probe is contacted with the sample under conditions in which the probe binds selectively with the target polynucleotide sequence to form a stable hybridization complex; and
detecting the formation of a hybridization complex.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to DNA sequences from regions of copy number change on chromosome 20. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases.
84 Citations
11 Claims
-
1. A method of screening for the presence of breast cancer cells in a sample, the method comprising:
-
contacting a nucleic acid sample from a human patient with a probe which binds selectively to a target polynucleotide sequence on a region in which copy number is altered in breast cancer cells and is selected from the group consisting of Flpter 0.603, 0.646, 0.675 0.694, 0.772 or 0.867 on chromosome 20, wherein the probe is contacted with the sample under conditions in which the probe binds selectively with the target polynucleotide sequence to form a stable hybridization complex; and
detecting the formation of a hybridization complex. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
-
11. A kit for the detection of a chromosome abnormality correlated with breast cancer, the kit comprising a compartment which contains more than one nucleic acid probe which binds selectively to a target polynucleotide sequence in a region of a chromosome correlated with breast cancer, wherein the probes bind selectively with the target polynucleotide sequence selected from the group consisting of Flpter 0.603, 0.646, 0.675, 0.694, 0.722 and 0.867 on chromosome 20.
Specification