×

Rapid thermocycling for sample analysis

  • US 6,210,882 B1
  • Filed: 01/29/1998
  • Issued: 04/03/2001
  • Est. Priority Date: 01/29/1998
  • Status: Expired due to Term
First Claim
Patent Images

1. A method for thermocycling a sample comprising:

  • (a) placing a volume of sample in the nanoliter range in a reaction vessel having a large surface to volume ratio;

    (b) heating the sample to a first desired temperature using the optical energy of a non-contact heat source that directly heats the sample itself at a rate of between about 10°

    C./second and 100°

    C./second;

    (c) positively cooling the sample to a second desired temperature by a non-contact cooling source below ambient temperature directed at the reaction vessel at a rate of between about 5°

    C./second and 50°

    C./second; and

    (d) sequentially repeating steps (b) and (c) to perform a desired number of thermocycles wherein each thermocycle is completed in between about 0.3 seconds and about 20 seconds.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×