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Rolling circle replication reporter systems

  • US 6,210,884 B1
  • Filed: 08/11/1998
  • Issued: 04/03/2001
  • Est. Priority Date: 11/21/1995
  • Status: Expired due to Term
First Claim
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1. A method of amplifying nucleic acid sequences, the method comprising,(a) mixing a rolling circle replication primer with one or more amplification target circles, to produce a primer-ATC mixture, and incubating the primer-ATC mixture under conditions that promote hybridization between the amplification target circles and the rolling circle replication primer in the primer-ATC mixture, wherein the amplification target circles each comprise a single-stranded, circular DNA molecule comprising a primer complement portion, and wherein the primer complement portion is complementary to the rolling circle replication primer, and wherein at least one of the amplification target circles is tethered to a specific binding molecule so that the amplification target circle can rotate freely, and (b) mixing DNA polymerase with the primer-ATC mixture, to produce a polymerase-ATC mixture, and incubating the polymerase-ATC mixture under conditions that promote replication of the amplification target circles, wherein replication of the amplification target circles results in the formation of tandem sequence DNA.

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