Method and apparatus for conducting an array of chemical reactions on a support surface
First Claim
1. A method for conducting chemical reactions between a solution of a chemical reactant and an array of functionalized binding sites on a support surface comprising adding the solution of chemical reactant to the functionalized binding site in an amount where the solution of chemical reactant at each binding site is separate from the solution of chemical reactant at other binding sites by surface tension.
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Accused Products
Abstract
The invention provides apparatus and methods for making arrays of functionalized binding sites on a support surface. The invention further provides apparatus and methods for sequencing oligonucleotides and for identifying the amino acid sequence of peptides that bind to biologically active macromolecules, by specifically binding biologically active macromolecules to arrays of peptides or peptide mimetics.
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Citations
19 Claims
- 1. A method for conducting chemical reactions between a solution of a chemical reactant and an array of functionalized binding sites on a support surface comprising adding the solution of chemical reactant to the functionalized binding site in an amount where the solution of chemical reactant at each binding site is separate from the solution of chemical reactant at other binding sites by surface tension.
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2. A method of making nucleic acid array plates comprising:
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a) coating a support surface with a positive or negative photoresist substance which is subsequently exposed to light and developed to create a pattern of a first exposed region and a photoresist coated region on the support;
b) reacting said first exposed region with a reagent to form a stable hydrophobic matrix on said first exposed region;
c) removing the remaining photoresist coat on said photoresisted coated region so as to form a second exposed region; and
d) adding at least a first nucleic acid coupling reagent to at least one area of said second exposed region on the nucleic acid array plate. - View Dependent Claims (5, 6, 8, 9, 18, 19)
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3. A method of making nucleic acid array plates comprising:
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a) coating a support surface with a positive or negative photoresist substance which is subsequently exposed to light and developed to create a pattern of a first exposed surface and a photoresist coated surface on the support;
b) reacting said first exposed surface with a reagent to form a stable hydrophobic matrix on said first exposed surface;
c) removing the photoresist coat on said photoresisted coated surface so as to form a second exposed surface;
d) reacting said second exposed surface with a second reagent so as to convert said second exposed surface to functionalized hydrophilic binding sites; and
e) adding at least a first nucleic acid coupling reagent to at least one of said functionalized hydrophilic binding sites. - View Dependent Claims (7)
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4. A method of making nucleic acid array plates comprising the step of forming a pattern of functionalized hydrophilic binding sites on a support surface, wherein said functionalized hydrophilic binding sites are chemically reactive to bind nucleic acids in an aqueous solution deposited thereon, and wherein said functionalized hydrophilic binding sites are more polar than their surrounding surface to cause separation of said aqueous solution at one binding site from aqueous solutions at adjacent binding sites.
Specification