Methods, kits, electrodes and compositions for assessing the level of an analyte of interest in fluid samples
First Claim
1. A potentiometric method for determining a concentration of an analyte of interest present in a fluid sample in concentration optionally below its linear range of detection, the method comprising the steps of:
- (a) adding to the sample a predetermined amount of the analyte of interest, such that a total concentration of the analyte of interest present in the sample is within the linear range of its detection;
(b) contacting an analyte selective electrode being selective to the analyte of interest and a reference electrode with the sample;
(c) measuring a signal representative of a potential drop between said analyte selective electrode and said reference electrode; and
(d) using said signal for determining the concentration of the analyte of interest present in the sample prior to the addition of the predetermined amount of the analyte of interest.
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Accused Products
Abstract
A potentiometric method for determining low concentration of an analyte of interest present in a fluid sample in a concentration optionally below its linear range of detection in a presence of at least one interfering substance of known concentration the method is effected by following the steps of (a) adding to the sample a predetermined amount of the analyte of interest, such that a total concentration of the analyte of interest present in the sample is within the linear range of its detection; (b) providing an analyte selective electrode being selective to the analyte of interest and a reference electrode both electrodes being equilibrated with a solution including the at least one interfering substance in concentration similar to said known concentration; (c) contacting the analyte selective electrode and the reference electrode with the sample; (d) measuring a signal representative of a potential drop between the analyte selective electrode and the reference electrode; and (e) using the signal for determining the concentration of the analyte of interest present in the fluid sample prior to the addition of the predetermined amount of the analyte of interest.
10 Citations
29 Claims
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1. A potentiometric method for determining a concentration of an analyte of interest present in a fluid sample in concentration optionally below its linear range of detection, the method comprising the steps of:
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(a) adding to the sample a predetermined amount of the analyte of interest, such that a total concentration of the analyte of interest present in the sample is within the linear range of its detection;
(b) contacting an analyte selective electrode being selective to the analyte of interest and a reference electrode with the sample;
(c) measuring a signal representative of a potential drop between said analyte selective electrode and said reference electrode; and
(d) using said signal for determining the concentration of the analyte of interest present in the sample prior to the addition of the predetermined amount of the analyte of interest. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A potentiometric method for determining a concentration of an analyte of interest present in a fluid sample in presence of at least one interfering substance of known concentration, the method comprising the steps of:
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(a) providing an analyte selective electrode being selective to the analyte of interest and a reference electrode, both electrodes being equilibrated with a solution including the at least one interfering substance in concentration similar to said known concentration;
(b) contacting said analyte selective electrode and said reference electrode with the sample;
(c) measuring a signal representative of a potential drop between said analyte selective electrode and said reference electrode; and
(d) using said signal for determining the concentration of the analyte of interest present in the sample. - View Dependent Claims (9, 10, 11)
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12. A potentiometric method for determining low concentration of an analyte of interest present in a fluid sample in a concentration optionally below its linear range of detection in presence of at least one interfering substance of known concentration, the method comprising the steps of:
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(a) adding to the sample a predetermined amount of the analyte of interest, such that a total concentration of the analyte of interest present in the sample is within the linear range of its detection;
(b) providing an analyte selective electrode being selective to the analyte of interest and a reference electrode both being equilibrated with a solution including the at least one interfering substance in concentration similar to said known concentration;
(c) contacting said analyte selective electrode and said reference electrode with the sample;
(d) measuring a signal representative of a potential drop between said analyte selective electrode and said reference electrode; and
(e) using said signal for determining the concentration of the analyte of interest present in the fluid sample prior to the addition of the predetermined amount of the analyte of interest. - View Dependent Claims (13, 14, 15, 16, 17, 18)
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- 19. A device useful in executing a potentiometric method for determining a concentration of an analyte of interest present in a fluid sample in concentration optionally below its linear range of detection by the potentiometric method, the device comprising a container including a predetermined amount of the analyte of interest, such that when the fluid sample is added to the container a total concentration of the analyte of interest present in the sample is within the linear range of its detection.
- 24. An electrode equilibrating and maintaining solution useful in equilibrating and maintaining an analyte selective electrode and a reference electrode, said analyte selective electrode and reference electrodes being designed to determine a concentration of an analyte of interest in a fluid sample including a known concentration of at least one interfering substance, the solution having a composition comprising the at least one interfering substance in a concentration similar to said known concentration of the at least one interfering substance in the sample.
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27. A kit useful in executing a potentiometric method for determining low concentration of an analyte of interest present in a fluid sample in a concentration optionally below its linear range of detection by the potentiometric method, the sample including a known concentration of at least one interfering substance, the kit comprising:
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(a) a first container including a predetermined amount of the analyte of interest, such that when the fluid sample is added to the container a total concentration of the analyte of interest present in the sample is within the linear range of its detection; and
(b) a second container including an equilibrating and maintaining solution for equilibrating and maintaining an analyte selective electrode and a reference electrode, said analyte selective electrode and reference electrodes being designed to determine the concentration of the analyte of interest in the fluid sample, said solution having a composition including the at least one interfering substance in concentration similar to the known concentration of the at least one interfering substance in the sample. - View Dependent Claims (28, 29)
(c) a third container containing a low concentration reference solution which includes a low concentration of the analyte to be determined; and
(d) a fourth container containing a high concentration reference solution which includes a high concentration of the analyte to be determined;
wherein said low concentration reference solution and said high concentration reference solution may be employed for calibration.
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29. The kit of claim 27, further comprising an additional amount of said equilibrating and maintaining solution, said additional amount employed for washing said electrode.
Specification