Use of a chemical sensor

• US 6,214,206 B1
• Filed: 06/19/1997
• Issued: 04/10/2001
• Est. Priority Date: 01/03/1995
• Status: Expired due to Term

• Current Assignee: Chemel AB (Biotech-IgG Equity AB)
• Original Assignee: Chemel AB (Biotech-IgG Equity AB)
• Inventors: Kriz, Dario
• Primary Examiner(s): Tung, T.
• Application Number:
  US08/860,117
• Time in Patent Office: 1,391 Days
• Field of Search: 204/415, 204/403, 205/777.5, 205/778, 205/782, 205/782.5, 205/783, 205/787, 205/787.5
• US Class Current: 205/778
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 33/143 containing sugar

Abstract

A chemical sensor (1) for selectively detecting an analyte in a solution as described. The sensor comprises a flow-through chamber (2), a selective membrane (3), a transducer means (4), an inlet (5) for a liquid flow containing a recognition element, and an outlet (6). There is also described a method of selectively detecting an analyte in a solution, wherein a recognition element is contacted with the solution containing the analyte via a selective membrane, said contact resulting in a response detectable by transducer means. The recognition element is injected into a flow, the flow is passed into a flow-through chamber comprising a transducer means and the selective membrane, where it is contacted with the analyte passing from the solution outside the selective membrane, whereby the recognition element and the analyte interact to provide a signal which is detected by the transducer means. The use of the chemical sensor for detecting of analyte(s) in a reactor system, a flow system or in an in vivo system is also described.

Claims

1. A method of selectively detecting analytes in a solution, said method comprising the steps of:

forming a flow-through chamber, the chamber comprising a selective two-sided membrane, a transducer, a flow inlet, and a flow outlet;

adapting a first side of said membrane for sequential contact with at least two analytes so that at least some of each analyte passes through the membrane;

flowing a buffer into the inlet so that it passes through the chamber and out the outlet;

serially injecting at least two recognition elements into the buffer so that they flow with the buffer into the inlet, through the chamber and out the outlet;

allowing a first of the analytes to pass through the membrane to undergo a first interaction with a recognition element;

in response to the first interaction, employing the transducer to generate a first signal proportional to the concentration of the first analyte in the solution;

allowing a second of the analytes to pass through the membrane to undergo a second interaction with a recognition element;

in response to the second interaction, employing the transducer to generate a second signal proportional to the concentration of the second analyte in the solution; and

between the first and second signals, employing the buffer to refresh the sensor.