Analyte assay using particulate labels
First Claim
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1. A method for specific detection of one or more analytes in a sample, comprising the steps of:
- specifically binding any said one or more analytes in said sample with at least one scattered-light detectable particle of a size between 1 and 500 nm inclusive, illuminating any said particles bound with said analytes with non-evanescent wave light under conditions which produce scattered light from said particle and in which light scattered from one or more said particles can be detected by a human eye with less than 500 times magnification and without electronic amplification, and detecting said light scattered by any said particles under said conditions as a measure of the presence of said one or more analytes, wherein said illuminating and said detecting are on opposite sides of a solid phase surface.
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Abstract
Method for specific detection of one or more analytes in a sample. The method includes specifically associating any one or more analytes in the sample with a scattered-light detectable particle, illuminating any particle associated with the analytes with light under conditions which produce scattered light from the particle and in which light scattered from one or more particles can be detected by a human eye with less than 500 times magnification and without electronic amplification. The method also includes detecting the light scattered by any such particles under those conditions as a measure of the presence of the analytes.
585 Citations
123 Claims
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1. A method for specific detection of one or more analytes in a sample, comprising the steps of:
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specifically binding any said one or more analytes in said sample with at least one scattered-light detectable particle of a size between 1 and 500 nm inclusive, illuminating any said particles bound with said analytes with non-evanescent wave light under conditions which produce scattered light from said particle and in which light scattered from one or more said particles can be detected by a human eye with less than 500 times magnification and without electronic amplification, and detecting said light scattered by any said particles under said conditions as a measure of the presence of said one or more analytes, wherein said illuminating and said detecting are on opposite sides of a solid phase surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122)
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123. A method for specific detection of one or more analytes in a sample, comprising the steps of:
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specifically binding any said one or more analytes in said sample with at least one scattered light detectable particles of a size between 1 and 500 nm inclusive, illuminating any said particles bound with said analytes with non-evanescent wave light under conditions which produce scattered light from said particle and in which light scattered from one or more said particles can be detected by a human eye with less than 500 times magnification and without electronic amplification, and detecting said light scattered by any said particles under said conditions as a measure of the presence of said one or more analytes, wherein said detecting is at an angle to the perpendicular to a solid phase surface less than the critical angle, and wherein said illuminating and said detecting are arranged to reduce non-specific background light thereby allowing said specific detection of said one or more analytes bound with said particles.
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Specification