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Analyte assay using particulate labels

  • US 6,214,560 B1
  • Filed: 04/18/1997
  • Issued: 04/10/2001
  • Est. Priority Date: 04/25/1996
  • Status: Expired due to Term
First Claim
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1. A method for specific detection of one or more analytes in a sample, comprising the steps of:

  • specifically binding any said one or more analytes in said sample with at least one scattered-light detectable particle of a size between 1 and 500 nm inclusive, illuminating any said particles bound with said analytes with non-evanescent wave light under conditions which produce scattered light from said particle and in which light scattered from one or more said particles can be detected by a human eye with less than 500 times magnification and without electronic amplification, and detecting said light scattered by any said particles under said conditions as a measure of the presence of said one or more analytes, wherein said illuminating and said detecting are on opposite sides of a solid phase surface.

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