Centrifugal fermentation process

• US 6,214,617 B1
• Filed: 12/31/1998
• Issued: 04/10/2001
• Est. Priority Date: 03/28/1995
• Status: Expired due to Term

• Current Assignee: Sartorius Stedim North America Inc. (Sartorius AG)
• Original Assignee: Kinetic Systems Incorporated (Kinetics Holding GmbH)
• Inventors: Herman, Heath H.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/224,645
• Time in Patent Office: 831 Days
• Field of Search: 435/285.1, 435/286.1, 435/286.5, 435/286.7, 435/289.1, 435/813, 435/41, 435/68.1, 435/70.1, 435/71.1, 435/171, 435/348, 435/366, 435/394, 435/410
• US Class Current: 435/394
• CPC Class Codes: C12M 23/34 Internal compartments or partitions
• CPC Class Codes: C12M 23/58 Reaction vessels connected in series or in parallel combinations of bioreactors with other apparatus, C12M43/00
• CPC Class Codes: C12M 33/10 by centrifugation centrifuges in general B04B; Cyclones cyclones in general B04C
• CPC Class Codes: C12M 35/04 Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
• CPC Class Codes: C12M 41/00 Means for regulation, monitoring, measurement or control, e.g. flow regulation controlling or regulating chemical, physical or physicochemical processes B01J19/0006; heating or cooling apparatus for laboratory use B01L7/00; electro optical investigation of individual particles, flow cytometers G01N15/14; automatic analysis G01N35/00; controlling or regulating in general G06N
• CPC Class Codes: C12M 41/40 of pressure

Abstract

The present invention comprises a novel culture methods and devices in which living cells or subcellular biocatalysts are immobilized by the opposition of forces. The immobilized cells or biocatalysts may be attached to support complexes that add to the resultant vector forces.

Claims

1. A method of producing secreted products from a biocatalyst comprising containing the biocatalyst in at least one chamber in a centrifugal force field wherein a continuous flow of a liquid acts to create a force which opposes the centrifugal force field and wherein a gravitational force contributes to the resultant vector summation of all forces acting on the biocatalyst, wherein the gravitational force, the centrifugal force and the opposing liquid force substantially immobilize the biocatalyst at a position in the chamber, the liquid is at hydraulic pressures greater than ambient pressure, and wherein no gaseous phase is present in said chamber, wherein the biocatalyst produces secreted products.

2. The method of claim 1, wherein containing the biocatalyst comprises a plurality of chambers.

3. The method of claim 1, wherein the liquid contains a dissolved gas.

4. The method of claim 1, wherein the biocatalyst is a cell.

5. The method of claim 1, wherein the biocatalyst is chosen from the group comprising a subcellular component, an enzyme complex, and an enzyme complex immobilized on a solid support.

6. The method of claim 1, wherein the biocatalyst is chosen from the group comprising prokaryotic cells, algae cells, plant cells, yeast cells, fungal cells, insect cells, reptile cells and mammalian cells.

7. The method of claim 1, wherein the liquid contains a dissolved gas chosen from the group comprising air, O₂, CO₂, methane, NH₃, NO₂, Ar, He, N₂ and H₂ or any mixture thereof.

8. A method of removing contaminants from a liquid comprising containing a biocatalyst in at least one chamber in a centrifugal force field wherein a continuous flow of the liquid acts to create a force which opposes the centrifugal force field and wherein a gravitational force contributes to the resultant vector summation of all forces acting on the biocatalyst, wherein the gravitational force, the centrifugal force and the opposing liquid force substantially immobilize the biocatalyst at a position in the chamber, the liquid is at hydraulic pressures greater than ambient pressure, and wherein no gaseous phase is present in said chamber, wherein the biocatalyst removes contaminants from the liquid.

9. The method of claim 8, wherein containing the biocatalyst comprises a plurality of chambers.

10. The method of claim 8, wherein the liquid is an aqueous solution.

11. The method of claim 8, wherein the liquid contains a dissolved gas.

12. The method of claim 8, wherein the liquid contains a dissolved gas chosen from the group comprising air, O₂, CO₂, methane, NH₃, NO₂, Ar, He, N₂ and H₂ or any mixture thereof.

13. The method of claim 8, wherein the biocatalyst is a cell.

14. The method of claim 8, wherein the biocatalyst is chosen from the group comprising a subcellular component, an enzyme complex, and an enzyme complex immobilized on a solid support.

15. The method of claim 8, wherein the biocatalyst is chosen from the group comprising prokaryotic cells, algae cells, plant cells, yeast cells, fungal cells, insect cells, reptile cells and mammalian cells.

16. The method of claim 8, wherein the contaminants are chosen from the group comprising heavy metals and nitrate ions.