Method of detection of carcinogenic human papillomavirus
First Claim
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1. A method for distinguishing between high risk strains 16 and 33 or 18 of human papillomavirus and low risk strains 6 and 11 of human papillomavirus in a sample of human cervical tissue comprising:
- a) obtaining DNA from the human cervical tissue sample, b) simultaneously or individually adding oligonucleotide primer pairs which are specific for the E6 region of i) HPV 16 and 33, ii) HPV 18 or iii) HPV 6 and 11, c) amplifying the HPV E6 region by polymerase chain reaction using the E6 specific primer pair(s) and d) detecting the presence or absence of amplification products specific for HPV16 and 33 or 18 or HPV 6 and 11 to thereby distinguish between the presence of high risk strains HPV 16 and 33 or 18 and low risk strains HPV 6 and 11.
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Abstract
High risk carcinogenic human types 16, 18 and 33 are distinguished from low risk human papillomavirus types 6 and 11 in a sample of human cervical tissue. A selected characteristic portion of the E6 region of the virus defined by specific oligonucleotide primers is amplified using a polymerase chain reaction. The presence or absence of the characteristic portion of the E6 region is detected by gel electrophoresis or using a labeled oligonucleotide probe.
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Citations
4 Claims
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1. A method for distinguishing between high risk strains 16 and 33 or 18 of human papillomavirus and low risk strains 6 and 11 of human papillomavirus in a sample of human cervical tissue comprising:
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a) obtaining DNA from the human cervical tissue sample, b) simultaneously or individually adding oligonucleotide primer pairs which are specific for the E6 region of i) HPV 16 and 33, ii) HPV 18 or iii) HPV 6 and 11, c) amplifying the HPV E6 region by polymerase chain reaction using the E6 specific primer pair(s) and d) detecting the presence or absence of amplification products specific for HPV16 and 33 or 18 or HPV 6 and 11 to thereby distinguish between the presence of high risk strains HPV 16 and 33 or 18 and low risk strains HPV 6 and 11. - View Dependent Claims (2, 3, 4)
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Current AssigneePolartechnics Ltd.
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Original AssigneeBiosearch International Pty. Ltd.
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InventorsNightingale, Brian, Morris, Brian James
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Primary Examiner(s)ARTHUR, LISA BENNETT
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Application NumberUS09/000,634Time in Patent Office1,204 DaysField of Search435/6, 435/91.2, 435/5US Class Current435/5CPC Class CodesC12Q 1/686 Polymerase chain reaction [...C12Q 1/708 for papilloma
