Anti-microbial proteins

• US 6,218,508 B1
• Filed: 04/17/1996
• Issued: 04/17/2001
• Est. Priority Date: 10/21/1993
• Status: Expired due to Fees

• Current Assignee: Syngenta Participations AG (China National Chemical Corporation)
• Original Assignee: Novartis Ag
• Inventors: Nielsen, Klaus Kristian, Mikkelsen, Jørn Dalgaard, Kragh, Karsten Matthias
• Primary Examiner(s): Brusca, John S.
• Assistant Examiner(s): McGarry, Sean
• Application Number:
  US08/632,511
• Time in Patent Office: 1,826 Days
• Field of Search: 435/69.1, 435/69.2, 435/375, 514/2, 530/300, 530/324, 530/344, 530/370
• US Class Current: 530/344
• CPC Class Codes: A01N 65/08 Magnoliopsida [dicotyledons]
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8282 for fungal resistance

Abstract

An anti-microbial protein comprising a peptide having the amino acid sequence: AA₁-AA₂-AA₃-Cys-AA₅-AA₆-AA₇-AA₈-AA₉-CYS-AA₁₁-AA₁₂-AA₁₃-AA₁₄-Cys-Cys-AA₁₇-AA₁₈-AA₁₉-AA₂₀-AA₂₁-Cys-AA₂₃-AA₂₄-AA₂₅-AA₂₆-AA₂₇-AA₂₈-Cys-AA₃₀. An anti-microbial protein having the sequence depicted in any one of SEQ ID Nos 1-3. Recombinant DNA encoding such proteins. A vector comprising such DNA which is expressible in plants and which is linked to a plant operable promoter and terminator. Plants transformed with such recombinant DNA; the progeny of such plants which contain the DNA stably incorporated and hereditable in a Mendelian manner, and/or the seeds of such plants or such progeny.

Claims

1. A purified protein having the sequence depicted in any one of SEQ ID Nos 1-3.

2. The protein according to claim 1, in combinations with at least one protein selected from the group consisting of those depicted in Seq. ID Nos. 4-6.

3. The protein according to claim 1, in combination with at least one further protein having herbicidal resistance, plant growth-promoting, anti-fungal, anti-bacterial, anti-viral and/or anti-nematode properties.

4. The protein derived from expression of DNA encoding a protein as claimed in claim 1.

5. An anti-microbial composition comprising one or more of the proteins as claimed in claim 1.

6. A process for combating fungi which comprises exposing the fungi to at least one protein claimed in claim 1.

7. An extraction process for obtaining anti-microbial proteins as claimed in claim 1, from organic material containing them comprising submitting the material to maceration and solvent extraction, characterized in that material is a microorganism or a plant.

8. A composition comprising the protein combination of claim 2.

9. A composition comprising the protein combination of claim 3.

10. A process for combating fungi comprising exposing the fungi to the protein combination of claim 2.

11. A process for combating fungi comprising exposing the fungi to the protein combination of claim 3.

12. The process of claim 6 further comprising exposing the fungi to at least two proteins having a sequence depicted in any one of SEQ ID Nos 1-3.