Patterned binding of functionalized microspheres for optical diffraction-based biosensors
First Claim
1. A method of detecting an analyte in a medium comprising:
- adding a diffraction enhancing element to the medium suspected of containing the analyte, wherein the diffraction enhancing element has a receptor material thereon that is specific for the analyte;
contacting the medium with a sensing device, the sensing device comprising;
a polymer film; and
an analyte-specific receptor layer printed in a pattern onto the polymer film wherein the analyte-specific receptor layer has a receptor material thereon that is specific for the analyte, and wherein the receptor layer is not a self-assembling monolayer;
transmitting a light through the polymer film; and
detecting presence of the analyte by detecting a pattern formed by diffraction of the transmitted light.
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Accused Products
Abstract
The present invention provides an inexpensive and sensitive system and method for detecting analytes present in a medium. The system comprises a diffraction enhancing element, such as functionalized microspheres, which are modified such that they are capable of binding with a target analyte. Additionally, the system comprises a polymer film, which may include a metal coating, upon which is printed a specific, predetermined pattern of a analyte-specific receptors. Upon attachment of a target analyte to select areas of the polymer film, either directly or with the diffraction enhancing element, diffraction of transmitted and/or reflected light occurs via the physical dimensions and defined, precise placement of the analyte. A diffraction image is produced which can be easily seen with the eye or, optionally, with a sensing device.
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Citations
41 Claims
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1. A method of detecting an analyte in a medium comprising:
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adding a diffraction enhancing element to the medium suspected of containing the analyte, wherein the diffraction enhancing element has a receptor material thereon that is specific for the analyte;
contacting the medium with a sensing device, the sensing device comprising;
a polymer film; and
an analyte-specific receptor layer printed in a pattern onto the polymer film wherein the analyte-specific receptor layer has a receptor material thereon that is specific for the analyte, and wherein the receptor layer is not a self-assembling monolayer;
transmitting a light through the polymer film; and
detecting presence of the analyte by detecting a pattern formed by diffraction of the transmitted light. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A method of detecting an analyte in a medium comprising:
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adding a diffraction enhancing element to the medium suspected of containing the analyte, wherein the diffraction enhancing element has a receptor material thereon that is specific for the analyte;
contacting the medium with a sensing device, the sensing device comprising;
a polymer film coated with metal; and
an analyte-specific receptor layer printed in a pattern onto the metal-coated polymer film wherein the analyte-specific receptor layer has a receptor material thereon that is specific for the analyte, and wherein the receptor layer is not a self-assembling monolayer;
reflecting a light source off a surface of the metal-coated polymer film; and
detecting presence of the analyte by detecting a pattern formed by diffraction of the reflected light. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
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41. A method of detecting an analyte in a medium comprising:
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adding a diffraction enhancing element to the medium suspected of containing the analyte, wherein the diffraction enhancing element has a receptor material thereon that is specific for the analyte;
contacting the medium with a sensing device, the sensing device comprising;
a polymer film coated with metal; and
an analyte-specific receptor layer printed in a pattern onto the metal-coated polymer film wherein the analyte-specific receptor layer has a receptor material thereon that is specific for the analyte, and wherein the receptor layer is not a self-assembling monolayer;
transmitting a light through the polymer film; and
detecting presence of the analyte by detecting a pattern formed by diffraction of the transmitted light.
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Specification