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Computer-based methods and systems for sequencing of individual nucleic acid molecules

  • US 6,221,592 B1
  • Filed: 10/20/1998
  • Issued: 04/24/2001
  • Est. Priority Date: 10/20/1998
  • Status: Expired due to Term
First Claim
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1. A method for imaging a single labeled nucleotide on an individual double stranded nucleic acid molecule, comprising:

  • (a) nicking a double stranded nucleic acid molecule elongated and fixed onto a surface so that the double stranded nucleic acid molecule remains accessible for enzymatic reactions with enzymes for the addition of a labeled nucleotide creating a nicked strand;

    (b) enzymatically adding a single nucleotide comprising a label; and

    (c) imaging the added label.

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