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DNA diagnostics based on mass spectrometry

  • US 6,221,605 B1
  • Filed: 02/15/2000
  • Issued: 04/24/2001
  • Est. Priority Date: 03/17/1995
  • Status: Expired due to Term
First Claim
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1. A process for detecting a target nucleic acid sequence present in a biological sample, comprising the steps of:

  • a) obtaining a target nucleic acid sequence from a biological sample;

    b) replicating the target nucleic acid sequence, thereby producing a replicated molecule;

    c) specifically digesting the replicated nucleic acid molecule using at least one appropriate nuclease, thereby producing digested fragments;

    d) immobilizing the digested fragments onto a solid support containing complementary capture nucleic acid sequences to produce immobilized fragments; and

    e) analyzing the immobilized fragments by mass spectrometry, wherein the determination of the molecular weight of the immobilized fragments provide information on the target nucleic acid sequence.

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