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Methods for solid-phase amplification of DNA template (SPADT) using multiarrays

  • US 6,221,635 B1
  • Filed: 05/06/1999
  • Issued: 04/24/2001
  • Est. Priority Date: 05/06/1999
  • Status: Active Grant
First Claim
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1. A method of detecting the presence or absence of a specific nucleic acid in a sample comprising DNA, said method comprising performing an amplification reaction, wherein a solid support on which a 5′

  • and a 3′

    primer are irreversibly bound and said DNA is absorbed such that it is reversibly bound, is incubated under amplification conditions, and determining whether said specific nucleic acid is amplified, wherein when said specific nucleic acid is amplified, said specific nucleic acid is present in said sample and when said specific nucleic acid is not amplified, said specific nucleic acid is not present in said sample.

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