Method for determining a nucleic acid
First Claim
1. A method for determining a nucleic acid in a sample comprisingbinding to the nucleic acid a probe molecule having a polymeric backbone different from the natural sugar phosphate backbone via base mediated hydrogen bonding, wherein an electron acceptor or an electron donor or an electron acceptor and an electron donor is covalently bound to said probe molecule at a non-terminal subunit of said polymeric backbone not bearing a nucleobase, inducing an electron transfer from said electron donor or to said elector acceptor and determining the occurrence of the electronic transfer as a measure of said nucleic acid.
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Abstract
New electron transfer moiety labeled nucleic acid analogue probes are provided that can be used in methods for determining nucleic acids in a sample. The new probes can be prepared using novel monomer subunits in a chemical synthesis route. The nucleic acids can be determined by binding the probe molecules to the nucleic acid and inducing electron transfer within the complex formed. The occurrence of the electron transfer is determined as a measure of the nucleic acid.
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Citations
19 Claims
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1. A method for determining a nucleic acid in a sample comprising
binding to the nucleic acid a probe molecule having a polymeric backbone different from the natural sugar phosphate backbone via base mediated hydrogen bonding, wherein an electron acceptor or an electron donor or an electron acceptor and an electron donor is covalently bound to said probe molecule at a non-terminal subunit of said polymeric backbone not bearing a nucleobase, inducing an electron transfer from said electron donor or to said elector acceptor and determining the occurrence of the electronic transfer as a measure of said nucleic acid.
Specification