Device for performing an assay, a method for manufacturing said device, and use of a membrane in the manufacture of said device

• US 6,225,131 B1
• Filed: 10/25/1999
• Issued: 05/01/2001
• Est. Priority Date: 07/11/1997
• Status: Expired due to Term

• Current Assignee: PamGene B.V. (VitrOmics Healthcare Holding BV)
• Original Assignee: Akzo Nobel N.V. (Nouryon BV)
• Inventors: Maria Kreuwel, Hermanus Johannes, van Damme, Hendrik Sibolt
• Primary Examiner(s): Chin, Christopher L.
• Assistant Examiner(s): Pham, Minh-Quan K.
• Application Number:
  US09/403,559
• Time in Patent Office: 554 Days
• Field of Search: 435/5, 435/6, 435/7.1, 435/287.1, 435/287.2, 435/810, 436/518, 436/524, 422/57, 422/58
• US Class Current: 436/524
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00511 Walls of reactor vessels
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/703 : Viruses associated with AIDS
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 33/551 : the carrier being inorganic
• CPC Class Codes: Y10S 435/81 : Packaged device or kit

Abstract

A device for performing an assay comprising a substrate having through-going channels that open out onto a surface for sample application, the channels being provided in at least one cross-sectional area with a first binding substance capable of binding a particular analyte, the substrate being an electrochemically manufactured metal oxide membrane and containing the first binding substance within the through-going channels.

Claims

1. A device for performing an assay, which device comprises a substrate having through-going channels, said channels opening out on a surface and being oriented essentially perpendicular to the surface for sample application, the channels in at least one area of the surface for sample application being provided with a first binding substance capable of binding to an analyte, wherein the substrate is an electrochemically manufactured metal oxide membrane and the first binding substance is within the through-going channels in the substrate.

2. The device according to claim 1, wherein the first binding substance is chosen from the group consisting of a nucleic acid probe, an antibody, an antigen, a receptor, a hapten and a ligand for a receptor.

3. The device according to claim 1, wherein the first binding substance is covalently bound to the substrate.

4. The device according to claim 1, wherein the metal oxide membrane is comprised of aluminium oxide.

5. A method of manufacturing a device according to claim 1, wherein the first binding substance is synthesised in situ.

6. The method according to claim 5, wherein a compound for synthesising the first binding substance is applied to a particular area using ink-jet technology.

7. The method according to claim 6, wherein the compound is applied using electrostatic attraction.

8. A method of manufacturing a device according to claim 1, wherein the first binding substance is applied to a particular area using ink-jet technology.

9. The method according to claim 8, wherein the first binding substance is applied using electrostatic attraction.

10. A kit comprising a device according to claim 1, and a detection means for determining whether binding has occurred between the first binding substance and the analyte.

11. The kit according to claim 10 wherein the detection means comprises a second binding substance provided with a label.

12. The kit according to claim 11, wherein the label is capable of inducing a color reaction or capable of bio- or chemo- or photoluminescence.

13. A method for the detection of an analyte in a sample, comprising the steps of

a) contacting the sample with a device according to claim 1,

b) allowing binding to take place between the first binding substance and the analyte to be detected, and

c) detecting whether binding has occurred between first binding substance and analyte.

14. The method of claim 13, wherein the analyte comprises nucleic acid.

15. The method of claim 14, wherein the nucleic acid is derivable from human immunodeficiency virus.