Seed-preferred promoters

• US 6,225,529 B1
• Filed: 08/19/1999
• Issued: 05/01/2001
• Est. Priority Date: 08/20/1998
• Status: Expired due to Term

• Current Assignee: Pioneer Hi Bred International Inc (Corteva, Inc.)
• Original Assignee: Pioneer Hi Bred International Inc (Corteva, Inc.)
• Inventors: Lappegard, Kathryn K., Martino-Catt, Susan J.
• Primary Examiner(s): Fox, David T.
• Assistant Examiner(s): IBRAHIM, MEDINA AHMED
• Application Number:
  US09/377,648
• Time in Patent Office: 621 Days
• Field of Search: 800/278, 800/281, 800/287, 800/320, 800/320.1, 800/320.2, 800/320.3, 800/317, 435/69.1, 435/320.1, 435/468, 435/419, 435/412, 536/23.1, 536/23.2, 536/24.1, 536/23.6
• US Class Current: 800/287
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8222 Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
• CPC Class Codes: C12N 15/8234 Seed-specific, e.g. embryo, endosperm
• CPC Class Codes: C12N 9/90 Isomerases (5.)

Abstract

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for seed-preferred promoters isolated from genes for Cim1 (cytokinin-induced message). cZ19B1 (maize 19 kDa zein); or mi1ps (myo-inositol-1-phosphate synthase). A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the seed-preferred promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.

Claims

1. An isolated promoter that is capable of driving transcription in a seed-preferred manner, wherein the promoter comprises a promoter natively associated with DNA coding for maize Cim 1 (cytokinin-induced message);

cZ19B1 (maize 19KDa zein);

or maize mi1ps (myo-inositol-1-phosphate synthase).

2. The isolated promoter of claim 1 wherein the promoter is natively associated with DNA coding for maize Cim1 (cytokinin-induced message).

3. The isolated promoter of claim 1 wherein the promoter is natively associated with DNA coding for cZ19B1 (maize 19 Kda zein).

4. The isolated promoter of claim 1 wherein the promoter is natively associated with DNA coding for maize mi1ps (myo-inositol-1-phosphate synthase).

5. An isolated promoter that is capable of driving transcription in a seed-preferred manner, wherein the promoter comprises a nucleotide sequence set forth in any one of SEQ ID NOS 1, 4, or 7.

6. The isolated promoter of claim 5 wherein the promoter comprises the nucleotide sequence set forth in SEQ ID NO 1.

7. The isolated promoter of claim 5 wherein the promoter comprises the nucleotide sequence set forth in SEQ ID NO 4.

8. The isolated promoter of claim 5 wherein the promoter comprises the nucleotide sequence set forth in SEQ ID NO 7.

9. An isolated promoter that is capable of driving transcription in a seed-preferred manner, wherein the promoter comprises a nucleotide sequence having at least 60% sequence identity to SEQ ID NO 1, at least 90% sequence identity to SEQ ID NO 4, or at least 60% sequence identity to SEQ ID NO 7, wherein the % sequence identity is based on the entire sequence and is determined by GAP version 10 analysis using default parameters.

10. The isolated promoter of claim 9 wherein the promoter comprises a nucleotide sequence having at least 60% sequence identity to SEQ ID NO 1 wherein the % sequence identity is based on the entire sequence and is determined by GAP version 10 analysis using default parameters.

11. The isolated promoter of claim 9 wherein the promoter comprises a nucleotide sequence having at least 90% sequence identity to SEQ ID NO 4 wherein the % sequence identity is based on the entire sequence and is determined by GAP version 10 analysis using default parameters.

12. The isolated promoter of claim 9 wherein the promoter comprises a nucleotide sequence having at least 60% sequence identity to SEQ ID NO 7 wherein the % sequence identity is based on the entire sequence and is determined by GAP version 10 analysis using default parameters.

13. An isolated promoter that is capable of driving transcription in a seed-preferred manner, wherein the promoter comprises a nucleotide sequence that hybridizes to any one of SEQ ID NOS:

1 or 7, under highly stringent conditions.

14. The isolated promoter of claim 13 wherein the promoter is a nucleotide sequence that hybridizes to SEQ ID NO 1 under highly stringent conditions.

15. The isolated promoter of claim 13 wherein the promoter is a nucleotide sequence that hybridizes to SEQ ID NO 7 under highly stringent conditions.

16. An expression cassette comprising a promoter and a first nucleotide sequence operably linked to the promoter, wherein the promoter is capable of initiating seed-preferred transcription of the first nucleotide sequence in a plant cell, wherein the promoter comprises a second nucleotide sequence natively associated with DNA coding for maize Cim 1 (cytokinin-induced message);

cZ19B1 (maize 19 KDa zein);

or maize mi1ps (myo-inositol-1-phosphate synthase).

17. An expression cassette comprising a promoter and a first nucleotide sequence operably linked to the promoter, wherein the promoter is capable of initiating seed-preferred transcription of the first nucleotide sequence in a plant cell, wherein the promoter comprises a second nucleotide sequence comprising the nucleotide sequence set forth in any one of SEQ ID NOS 1, 4, or 7.

18. An expression cassette comprising a promoter and a first nucleotide sequence operably linked to the promoter, wherein the promoter is capable of initiating seed-preferred transcription of the first nucleotide sequence in a plant cell, wherein the promoter comprises a second nucleotide sequence having at least 60% sequence identity to SEQ ID NO 1, at least 90% sequence identity to SEQ ID NO 4, or at least 60% sequence identity to SEQ ID NO 7, wherein the % sequence identity is based on the entire sequence and is determined by GAP version 10 analysis using default parameters.

19. An expression cassette comprising a promoter and a first nucleotide sequence operably linked to the promoter, wherein the promoter is capable of initiating seed-preferred transcription of the first nucleotide sequence in a plant cell, wherein the promoter comprises a second nucleotide sequence that hybridizes to any one of SEQ ID NOS:

1 or 7, under highly stringent conditions.

20. An isolated promoter that is capable of driving transcription in a seed-preferred manner, wherein the promoter comprises a nucleotide sequence selected from the group consisting of:

a) the nucleotide sequences set forth in any one of SEQ ID NOS 1,4, or 7;

b) nucleotide sequences having at least 60% sequence identity to SEQ ID NO 1, at least 90% sequence identity to SEQ ID NO 4, or at least 60% sequence identity to SEQ ID NO 7, wherein the % sequence identity is based on the entire sequence and is determined by GAP version 10 analysis using default parameters; and

c) a nucleotide sequence that hybridizes to any one of SEQ ID NOS;

1 or 7, under highly stringent conditions.

21. An expression cassette comprising a promoter and a first nucleotide sequence operably linked to the promoter, wherein the promoter is capable of initiating seed-preferred transcription of the first nucleotide sequence in a plant cell, wherein the promoter comprises a second nucleotide sequence selected from the group consisting of:

a) the nucleotide sequences set forth in any one of SEQ ID NOS 1,4, or 7;

b) nucleotide sequences having at least 60% sequence identity to SEQ ID NO 1, at least 90% sequence identity to SEQ ID NO 4, or at least 60% sequence identity to SEQ ID NO 7, wherein the % sequence identity is based on the entire sequence and is determined by GAP version 10 analysis using default parameters; and

c) a nucleotide sequence that hybridizes to any one of SEQ ID NOS;

1 or 7, under highly stringent conditions.

22. A transformation vector comprising an expression cassette, the expression cassette comprising a promoter and a first nucleotide sequence operably linked to the promoter, wherein the promoter is capable of initiating seed-preferred transcription of the first nucleotide sequence in a plant cell, wherein the promoter comprises a second nucleotide sequence selected from the group consisting of:

a) the nucleotide sequences set forth in any one of SEQ ID NOS 1,4, or 7;

b) nucleotide sequences having at least 60% sequence identity to SEQ ID NO 1, at least 90% sequence identity to SEQ ID NO 4, or at least 60% sequence identity to SEQ ID NO 7, wherein the % sequence identity is based on the entire sequence and is determined by GAP version 10 analysis using default parameters; and

c) a nucleotide sequence that hybridizes to any one of SEQ ID NOS;

1 or 7, under highly stringent conditions.

23. A plant stably transformed with an expression cassette comprising a promoter and a first nucleotide sequence operably linked to the promoter, wherein the promoter is capable of initiating seed-preferred transcription of the first nucleotide sequence in a plant cell, wherein the promoter comprises a second nucleotide sequence selected from the group consisting of:

a) the nucleotide sequences set forth in any one of SEQ ID NOS 1,4, or 7;

b) nucleotide sequences having at least 60% sequence identity to SEQ ID NO 1, at least 90% sequence identity to SEQ ID NO 4, or at least 60% sequence identity to SEQ ID NO 7, wherein the % sequence identity is based on the entire sequence and is determined by GAP version 10 analysis using default parameters; and

c) a nucleotide sequence that hybridizes to any one of SEQ ID NOS;

1 or 7, under highly stringent conditions.

24. The plant of claim 23, wherein said plant is a monocot.

25. The plant of claim 24, wherein said monocot is corn, wheat, rice, barley, sorghum, or rye.

26. Seed of the plant of claim 23.

27. A method for selectively expressing a nucleotide sequence in a plant seed, the method comprising transforming a plant cell with a transformation vector comprising an expression cassette, the expression cassette comprising a promoter and a first nucleotide sequence operably linked to the promoter, wherein the promoter is capable of initiating seed-preferred transcription of the first nucleotide sequence in a plant cell, wherein the promoter comprises a second nucleotide sequence selected from the group consisting of:

a) the nucleotide sequences set forth in any one of SEQ ID NOS 1,4, or 7;

b) nucleotide sequences having at least 60% sequence identity to SEQ ID NO 1, at least 90% sequence identity to SEQ ID NO 4, or at least 60% sequence identity to SEQ ID NO 7, wherein the % sequence identity is based on the entire sequence and is determined by GAP version 10 analysis using default parameters; and

c) a nucleotide sequence that hybridizes to any one of SEQ ID NOS;

1 or 7, under highly stringent conditions.

28. The method of claim 27 further comprising regenerating a stably transformed plant from said transformed plant cell;

wherein expression of said nucleotide sequences alters the phenotype of said plant seed.

29. The method of claim 27, wherein said heterologous nucleotide sequence encodes a gene product involved in fatty acid synthesis.

30. The method of claim 27, wherein said heterologous nucleotide sequence encodes a gene product having enhanced amino acid content.

31. A plant cell stably transformed with an expression cassette comprising a promoter and a first nucleotide sequence operably linked to the promoter, wherein the promoter is capable of initiating seed-preferred transcription of the first nucleotide sequence in a plant cell, wherein the promoter comprises a second nucleotide sequence selected from the group consisting of:

a) the nucleotide sequences set forth in any one of SEQ ID NOS 1,4, or 7;

b) nucleotide sequences having at least 60% sequence identity to SEQ ID NO 1, at least 90% sequence identity to SEQ ID NO 4, or at least 60% sequence identity to SEQ ID NO 7, wherein the % sequence identity is based on the entire sequence and is determined by GAP version 10 analysis using default parameters; and

c) a nucleotide sequence that hybridizes to any one of SEQ ID NOS;

1 or 7, under highly stringent conditions.

32. The plant cell of claim 31, wherein said plant cell is from a monocotyledonous plant.

33. The plant cell of claim 32, wherein said plant is corn, wheat, rice, barley, sorghum, or rye.