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Chip-based species identification and phenotypic characterization of microorganisms

  • US 6,228,575 B1
  • Filed: 02/07/1997
  • Issued: 05/08/2001
  • Est. Priority Date: 02/08/1996
  • Status: Expired due to Term
First Claim
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1. A method for assigning a first organism to a particular group or species, comprising:

  • (a) providing an array of oligonucleotides at known locations on a substrate, said array comprising probes complementary to reference DNA or RNA sequences from a second organism;

    (b) obtaining a first hybridization pattern by hybridizing a target nucleic acid sequence from the first organism to the array; and

    (c) comparing the first hybridization pattern of the first organism to a second hybridization pattern obtained by hybridizing a target nucleic acid sequence from the second organism to the array, wherein differences between the first and second hybridization patterns are indicative that the first organism is assigned to a particular group or species.

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