Method to select and transfect cell subpopulations
First Claim
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1. A method for transfecting a target cell with DNA and magnetically selecting the resulting cell from a test sample, comprising the steps of:
- providing magnetic particles coated with a ligand which specifically binds, either directly or indirectly, to said target cell, said magnetic particles being associated with a vector containing said DNA;
contacting a test sample containing said target cell with said magnetic particles under conditions forming a magnetically responsive particle-cell complex and effecting transfection of said target cell with said DNA via said vector; and
generating in the test sample a magnetic field gradient of sufficiently high intensity to separate magnetically the transfected target cells from said test sample.
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Abstract
A method for transfecting and separating cells is disclosed. The method comprises preparing magnetic particles coated with genetic material and a cell-specific ligand, and using the particles to transfect target cells. The target cells may then be separated from the non-target cells by using a magnetic field.
101 Citations
1 Claim
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1. A method for transfecting a target cell with DNA and magnetically selecting the resulting cell from a test sample, comprising the steps of:
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providing magnetic particles coated with a ligand which specifically binds, either directly or indirectly, to said target cell, said magnetic particles being associated with a vector containing said DNA;
contacting a test sample containing said target cell with said magnetic particles under conditions forming a magnetically responsive particle-cell complex and effecting transfection of said target cell with said DNA via said vector; and
generating in the test sample a magnetic field gradient of sufficiently high intensity to separate magnetically the transfected target cells from said test sample.
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Specification
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Current AssigneeJanssen Diagnostics, LLC (Johnson & Johnson)
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Original AssigneeImmunivest Corporation
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InventorsTerstappen, Leon W. M. M.
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Primary Examiner(s)Schwartzman, Robert A.
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Assistant Examiner(s)Larson, Thomas G.
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Application NumberUS09/230,394Time in Patent Office832 DaysField of Search242/450, 242/489, 435/6, 435/173.1, 435/173.9, 435/325, 435/375, 435/455, 435/458, 435/459, 435/470, 514/44US Class Current435/173.9CPC Class CodesC12N 15/87 Introduction of foreign gen...
