Enzymes with xylanase activity from aspergillus aculeatus

• US 6,228,630 B1
• Filed: 06/22/2000
• Issued: 05/08/2001
• Est. Priority Date: 03/10/1993
• Status: Expired due to Term

• Current Assignee: Novozymes A/S (Novozymes)
• Original Assignee: Novo Nordisk A/S
• Inventors: Andersen, Lene Nonboe, Mullertz, Anette, Dalb.o slashed.ge, Henrik, Jacobsen, Tina Sejersg.ang.ard, Christgau, Stephan, Kofod, Lene Venke, Heldt-Hansen, Hans Peter, Si, Joan Qi, Munk, Niels, Kauppinen, MArkus Sakari
• Primary Examiner(s): Nashed, Nashaat T.
• Application Number:
  US09/599,661
• Time in Patent Office: 320 Days
• Field of Search: 435/200, 536/23.2, 536/23.74
• US Class Current: 435/200
• CPC Class Codes: A21D 8/042 with enzymes
• CPC Class Codes: A23K 20/189 Enzymes
• CPC Class Codes: A23K 50/75 for poultry
• CPC Class Codes: A23L 2/04 Extraction of juices machines or apparatus for extracting juice A23N1/00, A47J19/00
• CPC Class Codes: A23L 29/06 Enzymes
• CPC Class Codes: A23L 7/107 Addition or treatment with enzymes not combined with fermentation with microorganisms
• CPC Class Codes: C12C 5/004 Enzymes
• CPC Class Codes: C12C 5/006 Beta-glucanase or functionally equivalent enzymes
• CPC Class Codes: C12C 7/28 After-treatment , e.g. sterilisation C12C11/00 takes precedence
• CPC Class Codes: C12G 1/00 Preparation of wine or sparkling wine
• CPC Class Codes: C12G 1/0203 by microbiological or enzymatic treatment
• CPC Class Codes: C12N 9/2482 Endo-1,4-beta-xylanase (3.2.1.8)
• CPC Class Codes: C12Y 302/01008 Endo-1,4-beta-xylanase (3.2.1.8)
• CPC Class Codes: C12Y 302/01032 Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
• CPC Class Codes: D21C 5/005 Treatment of cellulose-containing material with microorganisms or enzymes

Abstract

An enzyme preparation comprising (a) first enzyme with xylanase activity and (b) a second enzyme having cellulotytic, xylanolytic or pectinolytic activity and are supported by the specification.

Claims

1. An enzyme preparation comprising (a) an essentially pure enzym having xylanase activity, as measured by release of reducing sugars from birch xylan or by release of blue color from AZCL-Birch xylan, said enzyme having a pH optimum of 4-5 and a temperature optimum of 70-80°

C., which is encoded by a DNA sequence which hybridizes to a DNA sequence depicted in SEQ ID NO. 3 under the following conditions;

hybridizing in 5×

SSC, 5×

Denhardt'"'"'s solution, 50 mM sodium phosphate, pH 6.8 and 50 ug denatured sonicated calf thymus DNA for 18 hrs. at about 40°

C. followed by warming tbree times in 2×

SSC, 0.2% SDS at 40°

C. for 30 minutes and (b) a second enzyme having cellulolytic, xylanolytic or pectinolytic activity.

2. The enzyme preparation according to claim 1, wherein said essentially pure enzyme has a molecluar weight of about 56 kDa.

3. The enzyme preparation according to claim 1, wherein said essentially pure enzyme has an apparent pI in the range of about 4.5-4.7.

4. The enzyme preparation according to claim 1, wherein said essentially pure enzyme is derivable from a filamentous fungus or yeast.

5. The enzyme preparation according to claim 1, wherein said essentially pure enzyme is derivable from a fungal strain of Aspergilus, Trichoderma, Penicillium, Fusarium or Humicola.

6. The enzyme preparation according to claim 1, wherein said essentially pure enzyme is derivable from a strain of Asperigillus arculeatus, Aspergillus niger or Asperigillus oryzae.

7. The enzyme preparation according to claim 1 wherein said essentially pure enzyme is encoded by a DNA sequence isolated from a DNA library of Aspergillus aculeatus, CBS 101.43.

8. The enzyme preparation according to claim 1, wherein said second ensyme is producible from Aspergillus, Humicola or Trichoderma.

9. The enzyme preparation according to claim 1, wherein said second enyme is CELUZYME™

.

10. The enzyme preparation according to claim 1, wherein said second enzyme is CELLUCLAST™

.

11. An enzyme preparation comprising an enzyme having xylanase activity, as measured by release of reducing sugars from birch xylan or by release of blue color from AZCL-Birch xylan, said enzyme has a pH optimum of 4-5;

(b) a temperature optimum of 70-80°

C.;

(c) is encoded by a DNA sequence comprising thc partial DNA sequnce depicted in SEQ ID NO. 41;

(d) is derivable from a strain of Aspergillus and (b) a second enzyme having cellulytic, xylanolytic or pectinolytic activity.

12. The preparation according to claim 11, wherein said essentially pure enzyme has a molecular weight of about 56 kDa.

13. The enzyme preparation according to claim 11, wherein said essentially pure enzyme has an apparent pI in the range of about 4.5-4.7.

14. The enzyme preparation according to claim 11, wherein said essentially pure enzyme is derivable from a filamentous fungus or yeast.

15. The enzyme preparation according to claim 11, wherein said essentially pure enzyme is derivable from a fungal strain of Aspergillus, Trichoderma, Penicillum, Fusarium or Humicola.

16. The enzyme preparation according to claim 11, wherein said essentially pure enzym is derivable from a strain of Aspergillus aculeatus, Aspergillus niger or Aspergillus oryzae.

17. The enzyme preparation according to claim 11, wherein said essentially pure enzyme is encoded by a DNA sequence isolated from a DNA library of Aspergillus aculeatus, CBS 101.43.

18. The enzyme preparation according to claim 11, wherein said second enzyme is producible fom Aspergillus, Humicola or Trichoderma.

19. The enzyme preparation according to claim 11, wherein said second enzyme is CELLUZYME™

.

20. The enzyme preparation according to claim 11, wherein said second enzyme is CELLUCLAST™

.