Apparatus for mixing and separation employing magnetic particles
First Claim
1. An apparatus for mixing magnetic particles in, and separating magnetic particles from a liquid medium, the apparatus comprising:
- a magnetically permeable container having a wall or walls containing a liquid medium;
a quantity of magnetic particles located in the liquid medium in said container;
a magnet disposed outside the wall or walls of said container generating a magnetic field gradient inside the container in a portion of the liquid medium, defining a magnetic field cavity in the liquid medium;
means for continuously changing the relative angular position between said magnetic particles in said container and said magnet causing movement of said magnetic particles throughout the magnetic field cavity in the liquid medium; and
means for moving said magnet, concurrently with continuously changing the relative angular position between said magnetic particles in said container and said magnet, along the outside wall or walls of said container from one end of the liquid medium in said container to the other, causing the magnetic field cavity to move from one end of the liquid medium in said container to the other, while said magnetic particles are moving throughout the magnetic field cavity.
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Accused Products
Abstract
An apparatus for carrying out the affinity separation of a target substance from a liquid test medium by mixing magnetic particles having surface immobilized ligand or receptor within the test medium to promote an affinity binding reaction between the ligand and the target substance. The test medium with the magnetic particles in a suitable container is removably mounted in an apparatus that creates a magnetic field gradient in the test medium. This magnetic gradient is used to induce the magnetic particles to move, thereby effecting mixing. The mixing is achieved either by movement of a magnet relative to a stationary container or movement of the container relative to a stationary magnet. In either case, the magnetic particles experience a continuous angular position change with the magnet. Concurrently with the relative angular movement between the magnet and the magnetic particles, the magnet is also moved along the length of the container causing the magnetic field gradient to sweep the entire length of the container. After the desired time, sufficient for the affinity reaction to occur, movement of the magnetic gradient is ended, whereby the magnetic particles are immobilized on the inside wall of the container nearest to the magnetic source. The remaining test medium is removed while the magnetic particles are retained on the wall of the container. The test medium or the particles may then be subjected to further processing.
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Citations
9 Claims
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1. An apparatus for mixing magnetic particles in, and separating magnetic particles from a liquid medium, the apparatus comprising:
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a magnetically permeable container having a wall or walls containing a liquid medium;
a quantity of magnetic particles located in the liquid medium in said container;
a magnet disposed outside the wall or walls of said container generating a magnetic field gradient inside the container in a portion of the liquid medium, defining a magnetic field cavity in the liquid medium;
means for continuously changing the relative angular position between said magnetic particles in said container and said magnet causing movement of said magnetic particles throughout the magnetic field cavity in the liquid medium; and
means for moving said magnet, concurrently with continuously changing the relative angular position between said magnetic particles in said container and said magnet, along the outside wall or walls of said container from one end of the liquid medium in said container to the other, causing the magnetic field cavity to move from one end of the liquid medium in said container to the other, while said magnetic particles are moving throughout the magnetic field cavity. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification