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High throughput assay system

  • US 6,232,066 B1
  • Filed: 07/02/1998
  • Issued: 05/15/2001
  • Est. Priority Date: 12/19/1997
  • Status: Expired due to Term
First Claim
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1. A method of detecting at least one nucleic acid target, comprising contacting a sample which may comprise said target(s) with a nuclease protection fragment(s) specific for and which binds to said target(s), exposing the s ple to a nuclease effective to digest remaining single strand nucleic acid, and then contacting the resultant sample with a combination which comprises, before the addition of said sample,i) a surface comprising multiple spatially discrete regions, at least two of which are substantially identical, each region comprising ii) at least two different anchors, each in association with iii) a bifunctional linker which has a first portion that is specific for the anchor, and a second portion that comprises a probe which is specific for said nuclease protection fragment(s), under conditions effective for said nuclease protection fragment(s) to bind to said combination, and detecting said bound protection fragment(s).

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