Tenascin-C nucleic acid ligands
First Claim
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1. A nucleic acid ligand to tenascin-C identified according to the method comprising:
- a) contacting a candidate mixture of nucleic acids with tenascin-C, wherein nucleic acids having an increased affinity to tenascin-C relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture;
c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to tenascin-C, whereby a nucleic acid ligand of tenascin-C may be identified.
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Abstract
Methods are described for the identification and preparation of nucleic acid ligands to tenascin-C. Included in the invention are specific RNA ligands to tenascin-C identified by the SELEX method. Further included in the invention are methods for detecting the presence of a disease condition in a biological tissue in which tenascin-C is expressed.
21 Citations
14 Claims
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1. A nucleic acid ligand to tenascin-C identified according to the method comprising:
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a) contacting a candidate mixture of nucleic acids with tenascin-C, wherein nucleic acids having an increased affinity to tenascin-C relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture;
c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to tenascin-C, whereby a nucleic acid ligand of tenascin-C may be identified. - View Dependent Claims (2, 3, 4)
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- 5. A purified and isolated non-naturally occurring nucleic acid ligand to tenascin-C.
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10. A method of identifying nucleic acid ligands to tenascin-C, the method comprising:
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a) contacting a candidate mixture of nucleic acids with tenascin-C, wherein nucleic acids having an increased affinity to tenascin-C relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture;
c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to tenascin-C, whereby a nucleic acid ligand of tenascin-C may be identified. - View Dependent Claims (11, 12, 13, 14)
d) repeating steps a), b), and c).
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12. The method of claim 10 wherein said candidate mixture of nucleic acids is comprised of single stranded nucleic acids.
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13. The method of claim 12 wherein said single stranded nucleic acids are ribonucleic acids.
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14. The method of claim 13 wherein said nucleic acids are 2′
- -F (2′
- fluoro) modified ribonucleic acids.
- -F (2′
Specification