×

PCR method for nucleic acid quantification utilizing second or third order rate constants

  • US 6,232,079 B1
  • Filed: 08/09/2000
  • Issued: 05/15/2001
  • Est. Priority Date: 06/04/1996
  • Status: Expired due to Term
First Claim
Patent Images

1. A method of determining a concentration of an amplified product in a selected polymerase chain reaction mixture comprising(a) determining a second order rate constant for the amplified product at a selected temperature and reaction conditions by monitoring rate of hybridization of a known concentration of the amplified product;

  • (b) determining rate of annealing for an unknown concentration of the amplified product; and

    (c) calculating the concentration of the amplified product from the rate of annealing and the second order rate constant.

View all claims
  • 3 Assignments
Timeline View
Assignment View
    ×
    ×