Fourier detection of species migrating in a microchannel

• US 6,233,048 B1
• Filed: 12/08/1998
• Issued: 05/15/2001
• Est. Priority Date: 07/16/1996
• Status: Expired due to Term

• Current Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Holdings Incorporated
• Inventors: Parce, J Wallace
• Primary Examiner(s): Font, Frank G.
• Assistant Examiner(s): STAFIRA, MICHAEL PATRICK
• Application Number:
  US09/207,864
• Time in Patent Office: 889 Days
• Field of Search: 356/344, 356/318, 204/277.R, 204/182.8, 204/180.1, 250/461.2, 270/198.2, 270/198.3, 270/658, 270/656, 73/615.3, 73/233.9, 73/614.8
• US Class Current: 356/344
• CPC Class Codes: G01N 27/44721 by optical means
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: Y10S 366/02 Micromixers: segmented laminar flow with boundary mixing orthogonal to the direction of fluid propagation with or without geometry influences from the pathway

Abstract

The present invention provides a microfluidic system for fast, accurate and low cost electrophoretic analysis of materials in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields. Light from periodically spaced regions along a channel in the microfluidic system are received by a photodetector. The intensity of light received by the photodetector is modulated by the movement of species bands through the channel under electrophoretic forces. By Fourier analysis, the velocity of each species band is determined and the identification of the species is made by its electrophoretic mobility in the channel.

Claims

1. A method of analyzing subject materials, comprising:

transporting the subject materials through a microchannel and separating the subject materials into species bands;

detecting the species bands at a plurality of periodically spaced regions along the microchannel; and

analyzing frequencies at which the species bands are detected at the plurality of periodically spaced regions to determine a velocity of the species bands to analyze the subject materials.

2. The method of claim 1, wherein the subject materials comprise nucleic acids.

3. The method of claim 1, wherein the subject materials comprise proteins.

4. The method of claim 1, wherein the species bands comprise a fluorescent label, and the detecting step comprises exciting and detecting the fluorescent label.

5. The method of claim 1, wherein the detecting step comprises directing light at the periodically spaced regions and detecting a light signal from the microchannel.

6. The method of claim 1, wherein the detecting step comprises directing light at the microchannel and detecting a light signal from the plurality of periodically spaced regions.

7. The method of claim 1, wherein the analyzing step comprises analyzing the frequencies by Fourier analysis to determine the velocities of the species bands.

8. The method of claim 1, wherein the transporting step comprises electrophoretically transporting the subject materials through the microchannel.

9. The method of claim 1, wherein the microchannel disposed in a planar substrate.

10. A microfluidic system, comprising:

a microchannel configured to separate a subject material into species bands;

a detection system for detecting the species bands at a plurality of periodically spaced regions along the microchannel;

a frequency analyzer unit operably linked to the detection system for monitoring a frequency at which the species bands past the plurality of periodically spaced regions as detected by the detection system, and determining a velocity of the species bands based upon the frequency.

11. The microfluidic system of claim 10, wherein the microchannel comprises a channel disposed with a planar substrate.

12. The microfluidic system of claim 10, wherein the microchannel intersects at least a second microchannel disposed in the planar substrate.

13. The microfluidic system of claim 10, wherein the substrate is selected from glass and polymer substrates.

14. The microfluidic system of claim 10, wherein the microchannel comprises at least one cross-sectional dimension between about 0.1 and 100 μ

m.

15. The microfluidic system of claim 12, wherein the planar substrate comprises at least first and second planar substrate layers, the microchannel being disposed in a first surface of the first planar substrate, and the second substrate overlaying the first substrate to enclose and fluidly seal the microchannel.