Absorption information measuring method and apparatus of scattering medium

• US 6,233,470 B1
• Filed: 10/08/1999
• Issued: 05/15/2001
• Est. Priority Date: 08/30/1996
• Status: Expired due to Term

• Current Assignee: Hamamatsu Photonics KK
• Original Assignee: Hamamatsu Photonics KK
• Inventors: Tsuchiya, Yutaka
• Primary Examiner(s): WINAKUR, ERIC FRANK
• Application Number:
  US09/414,822
• Time in Patent Office: 585 Days
• Field of Search: 600/310, 600/322, 600/323, 600/330, 600/473, 600/476, 356/432, 356/433, 250/339.12, 250/341.1
• US Class Current: 600/310
• CPC Class Codes: G01N 21/49 within a body or fluid

Abstract

A method characterized by making modulated light having a predetermined modulation frequency component, incident to a scattering medium, receiving the modulated light having propagated inside the scattering medium to acquire measurement signals, detecting signals of the foregoing modulation frequency component from the measurement signals, obtaining amplitudes and inclinations of phase against modulation angular frequency, of the signals of the foregoing modulation frequency component, calculating a difference between absorption coefficients being primary information, based on a predetermined relation among the amplitudes, the inclinations of phase against modulation angular frequency and the difference between absorption coefficients, and calculating a difference of concentration of an absorptive constituent being secondary information, based on the difference between absorption coefficients.

Claims

1. A method of measuring absorption information of a scattering medium, said method comprising:

applying modulated light having a predetermined modulation frequency component to form a spot shape incident on a surface of a scattering medium comprising a measured object;

acquiring respective measurement signals by receiving said modulated light having propagated inside said measured object at one or both of a plurality of different times and a plurality of positions on said surface of said scattering medium;

detecting, from each of said respective measurement signals, respective signals of said modulation frequency component;

obtaining, from said respective signals of said modulation frequency component, amplitude components and phase component slopes against modulation angular frequency; and

calculating a difference between absorption coefficients of said measured object based upon a predetermined relation among said amplitude components, said phase component slopes against modulation angular frequency, and a difference between absorption coefficients corresponding to different ones of said respective measurement signals.

2. A method according to claim 1, wherein a difference of concentration of an absorptive constituent is quantified based upon a predetermined relation among said difference between absorption coefficients, an absorption coefficient per unit concentration of an absorptive constituent, and said difference of concentration of said absorptive constituent.

3. A method of measuring absorption information of a scattering medium, said method comprising:

applying a plurality of modulated light beams to form a spot shape incident on a surface of a scattering medium comprising a measured object, each of said plurality of modulated light beams having a predetermined modulation frequency component and a different wavelength at which scattering coefficients of said measured object are substantially equal to one another;

acquiring respective measurement signals for said different wavelengths by receiving said modulated light beams having propagated inside said measured object at a predetermined position on said surface of said scattering medium;

detecting, from each of said respective measurement signals, respective signals of said modulation frequency component;

obtaining, from said respective signals of said modulation frequency component, amplitude components and phase component slopes against modulation angular frequency; and

calculating a difference between absorption coefficients of said measuring object at said respective wavelengths based upon a predetermined relation among said amplitude components, said phase component slopes against modulation angular frequency, and said difference between absorption coefficients of said measured object at said respective wavelengths.

4. The method according to claim 3, further comprising quantifying a concentration of the absorptive constituent based upon a predetermined relation among said difference between absorption coefficients, absorption coefficients per unit concentration of an absorptive constituent at said respective wavelengths, and said concentration of said absorptive constituent.

5. The method according to claim 3, wherein said measurement signals comprise a plurality of measurement signals obtained when said light beams are received at a plurality of different positions in said surface of said scattering medium.

6. An apparatus for measuring absorption information of a scattering medium, said apparatus comprising:

a light source, configured and arranged to apply modulated light having a predetermined modulation frequency component to form a spot shaped incident on a surface of a scattering medium comprising a measured object;

a photodetector, configured and arranged to acquire respective measurement signals by receiving said modulated light having propagated inside said measured object at one or both of a plurality of different times and a plurality of positions on said surface of said scattering medium;

a signal detector, configured and arranged to detect, from said respective measurement signals, respective signals of said modulation frequency component;

a first arithmetic section, configured and arranged to calculate, from said respective signals of said modulation frequency component, amplitude components and phase component slopes against modulation angular frequency; and

a second arithmetic section for calculating a difference between absorption coefficients of said measuring object based upon a predetermined relation among said amplitude components, said phase component slopes against modulation angular frequency, and said difference between absorption coefficients of said measuring object.

7. The apparatus according to claim 6, wherein said second arithmetic section further is configured and arranged to calculate a difference of concentration of an absorptive constituent based upon a predetermined relation among said difference between absorption coefficients, an absorption coefficient per unit concentration of said absorptive constituent, and said difference of concentration of said absorptive constituent.

8. An apparatus for measuring absorption information of a scattering medium, said apparatus comprising:

a light source, configured and arranged to apply a plurality of modulated light beams to form a spot shaped incident on a surface of a scattering medium comprising a measured object, each of said plurality of modulated light beams having a predetermined modulation frequency component and having a predetermined different wavelength at which scattering coefficients of said measured object are substantially equal to one another;

a photodetector, configured and arranged to acquire respective measurement signals for said different wavelengths by receiving said modulated light beams having propagated inside said measured object at a predetermined position in said surface of said scattering medium;

a signal detector, configured and arranged to detect, from said respective measurement signals, respective signals of said modulation frequency component;

a first arithmetic section for obtaining, from said respective signals of said modulation frequency component, amplitude components and phase component slopes against modulation angular frequency; and

a second arithmetic section for calculating a difference between absorption coefficients of said measuring object based upon a predetermined relation among said amplitude components, said phase component slopes against modulation angular frequency, and said difference between absorption coefficients.

9. The apparatus according to claim 8, wherein said second arithmetic section further quantifies a concentration of an absorptive constituent based upon a predetermined relation among said difference between absorption coefficients, absorption coefficients per unit concentration of an absorptive constituent at said different wavelengths, and said concentration of said absorptive constituent.

10. The apparatus according to claim 8, wherein said photodetector comprises a light receiver capable of receiving said light beams at a plurality of positions in said surface of said scattering medium, and wherein said measurement signals comprise a plurality of measurement signals obtained when said light beams are received at a plurality of positions in said surface of said scattering medium.