Nucleic acid detecting reagent

• US 6,235,472 B1
• Filed: 06/18/1998
• Issued: 05/22/2001
• Est. Priority Date: 02/16/1994
• Status: Expired due to Term

• Current Assignee: Amersham Pharmacia Biotech AB
• Original Assignee: Amersham Pharmacia Biotech AB
• Inventors: Kwiatkowski, Marek, Landegren, Ulf
• Primary Examiner(s): ARTHUR, LISA BENNETT
• Application Number:
  US09/099,466
• Time in Patent Office: 1,069 Days
• Field of Search: 435/6, 435/195, 536/24.3, 536/24.31, 536/24.32
• US Class Current: 435/6.12
• CPC Class Codes: C12Q 1/6813 Hybridisation assays

Abstract

The present invention provides a nucleic acid detecting agent able to interact with a target sequence through catenation. The invention provides a nucleic acid detecting reagent comprising a) two free nucleic acid ends which are at least partially complementary to and capable of hybridizing to two adjacent regions of a target nucleic acid sequence; and b) an intermediate segment between the ends, the intermediate segment being labeled with a least one detectable element selected from the group consisting of chromophores, fluorophores, luminescers, radiolabels, affinity groups and enzymes, wherein only the ends of the reagent hybridize to the target nucleic acid sequence.

Claims

1. A nucleic acid detecting reagent comprising

a) two free nucleic acid ends which are at least partially complementary to and capable of hybridizing to two adjacent regions of a target nucleic acid sequence;

and

b) an intermediate segment between said ends, said intermediate segment being labelled with a least one detectable element selected from the group consisting of chromophores, fluorophores, luminescers, radiolabels, affinity groups and enzymes,

wherein only said ends of said reagent hybridize to said target nucleic acid sequence.

2. The nucleic acid detecting reagent according to claim 1, wherein said intermediate segment comprises a non-nucleic acid material selected from the group consisting of protein, polypeptide, carbohydrate, and a synthetic polymer.

3. The nucleic acid detecting reagent according to claim 1, wherein after hybridization of said ends and covalent connection of said ends, said reagent becomes circularized and catenates with said target nucleic acid sequence.

4. The nucleic acid detecting reagent according to claim 2, wherein after hybridization of said ends and covalent connection of said ends, said reagent becomes circularized and catenates with said target nucleic acid sequence.

5. A reagent kit for detecting a target nucleic acid sequence, said kit comprising the reagent according to claim 1, and a ligating means for connecting said reagent ends to each other after said reagent has hybridized to said target sequence.

6. A reagent kit for detecting a target nucleic acid sequence, said kit comprising the reagent according to claim 2, and a ligating means for connecting said reagent ends to each other after said reagent has hybridized to said target sequence.

7. A reagent kit for detecting a target nucleic acid sequence, said kit comprising the reagent according to claim 3, and a ligating means for connecting said reagent ends to each other after said reagent has hybridized to said target sequence.

8. A reagent kit for detecting a target nucleic acid sequence, said kit comprising the reagent according to claim 4, and a ligating means for connecting said reagent ends to each other after said reagent has hybridized to said target sequence.

9. The reagent kit according to claim 5, wherein said ligating means is a ligase.

10. The reagent kit according to claim 6, wherein said ligating means is a ligase.

11. The reagent kit according to claim 7, wherein said ligating means is a ligase.

12. The reagent kit according to claim 8, wherein said ligating means is a ligase.