Method for multi-spectral analysis of organic blood analytes in noninvasive infrared spectroscopy

• US 6,236,047 B1
• Filed: 08/27/1998
• Issued: 05/22/2001
• Est. Priority Date: 02/02/1996
• Status: Expired due to Term

• Current Assignee: GLT Acquisition Corporation
• Original Assignee: Instrumentation Metrics Inc.
• Inventors: Malin, Stephen F., Khalil, Gamal
• Primary Examiner(s): Ham, Seungsook
• Assistant Examiner(s): HANIG, RICHARD E
• Application Number:
  US09/141,283
• Time in Patent Office: 999 Days
• Field of Search: 250/339.12, 250/339.09, 250/339.11, 250/341.8, 600/316, 600/322, 600/347, 600/365
• US Class Current: 250/339.12
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/725 using specific filters therefor, e.g. Kalman or adaptive filters A61B5/316 take precedence
• CPC Class Codes: A61B 5/7253 characterised by using transforms
• CPC Class Codes: G01N 21/314 with comparison of measurements at specific and non-specific wavelengths dual wavelength spectrometry G01J3/427
• CPC Class Codes: G01N 21/359 using near infrared light

Abstract

A method is described for determining the concentration of an organic blood analyte using multi-spectral analysis in the near infrared and mid-infrared ranges. Incident radiation containing a plurality of distinct, nonoverlapping regions of wavelengths in the range of approximately 1100 to 5000 nm is used to scan a sample. Diffusively reflected radiation emerging from the sample is detected, and a value indicative of the concentration of the analyte is obtained using an application of chemometrics techniques. Information obtained from each nonoverlapping region of wavelengths can be cross-correlated in order to remove background interferences.

Claims

1. A method of determining the concentration of an organic blood analyte in a body tissue sample, comprising:

(a) selecting a plurality of distinct, nonoverlapping spectral regions within an infrared spectrum, wherein each of the spectral regions encompass a range of wavelengths between approximately 1100 and 5000 nanometers, and wherein at least a portion of each spectral region has high correlation to the concentration of the analyte;

(b) irradiating the sample with light having a wavelength in each spectral region to obtain radiation reflected from and modified by contact with the sample in each spectral region;

(c) optically filtering the reflected modified radiation to isolate or emphasize a portion of the radiation from each spectral region;

(d) collecting and measuring the intensity of the optically filtered radiation using a detector means; and

(e) obtaining a value indicative of the analyte concentration by applying a mathematical model to the optically filtered radiation.

2. The method of claim 1, wherein step (c) comprises passing the modified radiation through optical filter means having absorption characteristics capable of selectively passing discrete wavelengths from each spectral region, wherein said discrete wavelengths are specifically correlated with the concentration of the analyte.

3. The method of claim 2, wherein the absorption characteristics of the optical filter means are derived using chemometrics techniques.

4. The method of claim 1, wherein the detector means comprises a plurality of detectors.

5. The method of claim 4, wherein the plurality of detectors comprise lead selenide (PbSe) detectors.

6. The method of claim 1, wherein the organic blood analyte is selected from the group consisting of glucose, urea (BUN), lipids, bilirubin and ethyl alcohol.

7. The method of claim 6, wherein the blood analyte is glucose.

8. A method of determining the concentration of an organic blood analyte in a body tissue sample, comprising:

(a) selecting a plurality of distinct, nonoverlapping spectral regions within an infrared spectrum, wherein each of the spectral regions encompass a range of wavelengths between approximately 1100 and 5000 nanometers, and wherein at least a portion of each spectral region has high correlation to the concentration of the analyte;

(b) irradiating the sample with incident light having a wavelength in each spectral region to obtain radiation reflected from and attenuated by contact with the sample and radiation unattenuated by contact with the sample, wherein the attenuated radiation is contained within each spectral region;

(c) collecting the reflected attenuated radiation;

(d) measuring the intensity of the collected reflected attenuated radiation at a predetermined wavelength in each of the spectral regions; and

(e) correlating the intensity measurements obtained in step (d) to determine a value indicative of the concentration of the analyte.

9. The method of claim 8, wherein the infrared spectrum contains a first spectral region of wavelengths in the range of approximately 1100 to 1350 nm, a second spectral region of wavelengths in the range of approximately 1550 to 1850 nm, and a third spectral region of wavelengths in the range of approximately 2000 to 3500 nm.

10. The method of claim 9, further comprising the steps of collecting and measuring the intensity of the unattenuated radiation, wherein said unattenuated radiation is from the first spectral region and contains wavelengths in the range of approximately 1320 to 1340 nm.

11. The method of claim 10, wherein the measure of the intensity of the unattenuated radiation is used to estimate the intensity of the incident light used in step (b).

12. The method of claim 10, wherein said reflected attenuated radiation includes highly attenuated radiation and wherein the highly attenuated radiation encompasses a band of wavelengths corresponding to a peak in the infrared absorption of water.

13. The method of claim 12, further comprising the steps of collecting and measuring the intensity of the highly attenuated radiation.

14. The method of claim 13, wherein the measure of the intensity of the highly attenuated radiation is used to estimate the intensity of the light not absorbed by the irradiated sample.

15. The method of claim 14, wherein the band of wavelengths corresponding to a peak in the infrared absorption spectrum of water is in the range of approximately 1430 to 1450 nm.

16. The method of claim 14, wherein the band of wavelengths corresponding to a peak in the infrared absorption spectrum of water is in the range of approximately 1930 to 1950 nm.