Optical system for a scanning fluorometer

• US 6,236,456 B1
• Filed: 06/21/1999
• Issued: 05/22/2001
• Est. Priority Date: 08/18/1998
• Status: Expired due to Term

• Current Assignee: Molecular Devices
• Original Assignee: Molecular Devices
• Inventors: Kaye, Roger, Hafeman, Dean, Ogle, David G., Giebeler, Robert
• Primary Examiner(s): EVANS, FANNIE L
• Application Number:
  US09/337,623
• Time in Patent Office: 701 Days
• Field of Search: 356/317, 356/318, 356/417, 250/458.1, 250/459.1, 250/461.1, 250/461.2, 422/82.08, 436/172
• US Class Current: 356/318
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 2021/6471 Special filters, filter wheel
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Abstract

A method and apparatus for determining the fluorescence, luminescence, or absorption of a sample is provided. The sample may either be contained within a cuvette or within one or more sample wells within a multi-assay plate. A combination of a broadband source, a monochromator, and a series of optical filters are used to tune the excitation wavelength to a predetermined value within a relatively wide wavelength band. A similar optical configuration is used to tune the detection wavelength. In one aspect, multiple optical fibers are coupled to the excitation source subassembly, thus allowing the system to be quickly converted from one optical configuration to another. For example, the source can be used to illuminate either the top or the bottom of a sample well within a multi-assay plate or to illuminate a single cuvette cell. Similarly, multiple optical fibers are coupled to the detector subassembly. In another aspect, the excitation light and the detected sample emissions pass to and from an optical head assembly via a pair of optical fibers. The optical head assembly is scanned across one axis of the sample multi-assay plate. The multi-assay plate is mounted to a carriage assembly that scans the plate along a second axis orthogonal to the first axis. In another aspect, an optical scanning head assembly is used that includes mirrored optics for coupling the excitation source to the sample and the emitted light to the detector.

Claims

1. A sample characterizing instrument, comprising:

a source of excitation light comprising;

a lamp;

a first scanning monochromator; and

a plurality of bandpass filters within a first filter wheel, wherein a bandpass associated with each of said plurality of bandpass filters is between about 10 and about 100 nanometers;

a sample testing region;

a detection assembly comprising;

a detector;

a second scanning monochromator; and

a plurality of optical filters within a second filter wheel;

an optical assembly coupled to said excitation light source and coupled to said detection assembly, wherein said optical assembly directs said excitation light to said sample testing region, and wherein said optical assembly directs sample light from said sample testing region to said detection assembly;

a carriage assembly coupled to said optical assembly and to said sample testing region, said carriage assembly controlling the relative movement of said sample testing region and said optical assembly to provide scanning of at least a portion of said sample testing region; and

a processor coupled to said carriage assembly, said excitation light source, and said detection assembly, wherein said processor controls said scanning of said carriage assembly along a first scanning axis and a second scanning axis, wherein said processor controls an output wavelength of said excitation light source by controlling said lamp, said first scanning monochromator, and said plurality of bandpass filters, and wherein said processor controls a detection wavelength of said detector by controlling said second scanning monochromator and said plurality of optical filters.

2. The sample characterizing instrument of claim 1, wherein said bandpass associated with each of said plurality of bandpass filters is between about 40 and about 80 nanometers.

3. The sample characterizing instrument of claim 1, wherein a long wavelength cutoff value corresponding to each of said plurality of bandpass filters increases by predetermined steps of about 10 to about 35 nanometers.

4. The sample characterizing instrument of claim 1, wherein a long wavelength cutoff value corresponding to each of said plurality of bandpass filters increases by predetermined steps of about 35 to about 80 nanometers.

5. The sample characterizing instrument of claim 1, wherein said plurality of bandpass filters are selected from the group consisting of interference filters, colored glass filters, or Wrattan filters.

6. The sample characterizing instrument of claim 1, said optical assembly further comprising:

a first reflector for directing said excitation light toward said sample testing region;

a second reflector for reflecting sample light from said sample testing region, said reflected sample light detected by said detection assembly; and

an aperture within said second reflector, wherein said excitation light directed by said first reflector passes through said aperture prior to impinging on said sample testing region.

7. The sample characterizing instrument of claim 6, wherein a first optical axis corresponding to said second reflector is offset by a first angle away from a normal to a bottom surface of said sample testing region, and wherein a second optical axis corresponding to said first reflector is offset by a second angle away from said bottom surface normal.

8. The sample characterizing instrument of claim 6, wherein said first reflector and said second reflector are elliptical mirrors.

9. The sample characterizing instrument of claim 1, further comprising a first optical fiber coupling said excitation light source to said optical assembly and a second optical fiber coupling said detection assembly to said optical assembly.

10. The sample characterizing instrument of claim 1, further comprising:

a heater;

a temperature monitor;

an air circulation system; and

an enclosure substantially surrounding a sample plate holding fixture within said sample testing region for use with a multi-assay plate, wherein a humidity within said enclosure is in excess of 90 percent.

11. A sample characterizing instrument, comprising:

a source of excitation light comprising;

a lamp;

a first scanning monochromator; and

a second scanning monochromator;

a sample testing region;

a detection assembly comprising;

a detector;

a third scanning monochromator; and

a first plurality of optical filters within a first filter wheel;

an optical assembly coupled to said excitation light source and coupled to said detection assembly, wherein said optical assembly directs said excitation light to said sample testing region, and wherein said optical assembly directs sample light from said sample testing region to said detection assembly;

a carriage assembly coupled to said optical assembly and to said sample testing region, said carriage assembly controlling the relative movement of said sample testing region and said optical assembly to provide scanning of at least a portion of said sample testing region; and

a processor coupled to said carriage assembly, said excitation light source, and said detection assembly, wherein said processor controls said scanning of said carriage assembly along a first scanning axis and a second scanning axis, wherein said processor controls an output wavelength of said excitation light source by controlling said lamp, said first scanning monochromator, and said second scanning monochromator, and wherein said processor controls a detection wavelength of said detector by controlling said third scanning monochromator and said plurality of optical filters.

12. The sample characterizing instrument of claim 11, said excitation light source further comprising a second plurality of optical filters within a second filter wheel.

13. The sample characterizing instrument of claim 11, said optical assembly further comprising:

a first reflector for directing said excitation light toward said sample testing region;

a second reflector for reflecting sample light from said sample testing region, said reflected sample light detected by said detection assembly; and

an aperture within said second reflector, wherein said excitation light directed by said first reflector passes through said aperture prior to impinging on said sample testing region.

14. The sample characterizing instrument of claim 13, wherein a first optical axis corresponding to said second reflector is offset by a first angle away from a normal to a bottom surface of said sample testing region, and wherein a second optical axis corresponding to said first reflector is offset by a second angle away from said bottom surface normal.

15. The sample characterizing instrument of claim 13, wherein said first reflector and said second reflector are elliptical mirrors.

16. The sample characterizing instrument of claim 11, further comprising a first optical fiber coupling said excitation light source to said optical assembly and a second optical fiber coupling said detection assembly to said optical assembly.

17. The sample characterizing instrument of claim 11, further comprising:

a heater;

a temperature monitor;

an air circulation system; and

an enclosure substantially surrounding a sample plate holding fixture within said sample testing region for use with a multi-assay plate, wherein a humidity within said enclosure is in excess of 90 percent.

18. A sample characterizing instrument, comprising:

a source of excitation light comprising;

a lamp;

a first scanning monochromator; and

a first plurality of optical filters within a first filter wheel;

a sample testing region;

a detection assembly comprising;

a detector;

a second scanning monochromator; and

a third scanning monochromator;

an optical assembly coupled to said excitation light source and coupled to said detection assembly, wherein said optical assembly directs said excitation light to said sample testing region, and wherein said optical assembly directs sample light from said sample testing region to said detection assembly;

a carriage assembly coupled to said optical assembly and to said sample testing region, said carriage assembly controlling the relative movement of said sample testing region and said optical assembly to provide scanning of at least a portion of said sample testing region; and

a processor coupled to said carriage assembly, said excitation light source, and said detection assembly, wherein said processor controls said scanning of said carriage assembly along a first scanning axis and a second scanning axis, wherein said processor controls an output wavelength of said excitation light source by controlling said lamp, said first scanning monochromator, and said first plurality of optical filters, and wherein said processor controls a detection wavelength of said detector by controlling said second scanning monochromator and said third scanning monochromator.

19. The sample characterizing instrument of claim 18, said detection assembly further comprising a second plurality of optical filters within a second filter wheel.

20. The sample characterizing instrument of claim 18, said optical assembly further comprising:

a first reflector for directing said excitation light toward said sample testing region;

a second reflector for reflecting sample light from said sample testing region, said reflected sample light detected by said detection assembly; and

an aperture within said second reflector, wherein said excitation light directed by said first reflector passes through said aperture prior to impinging on said sample testing region.

21. The sample characterizing instrument of claim 20, wherein a first optical axis corresponding to said second reflector is offset by a first angle away from a normal to a bottom surface of said sample testing region, and wherein a second optical axis corresponding to said first reflector is offset by a second angle away from said bottom surface normal.

22. The sample characterizing instrument of claim 20, wherein said first reflector and said second reflector are elliptical mirrors.

23. The sample characterizing instrument of claim 18, further comprising a first optical fiber coupling said excitation light source to said optical assembly and a second optical fiber coupling said detection assembly to said optical assembly.

24. The sample characterizing instrument of claim 18, further comprising:

a heater;

a temperature monitor;

an air circulation system; and

an enclosure substantially surrounding a sample plate holding fixture within said sample testing region for use with a multi-assay plate, wherein a humidity within said enclosure is in excess of 90 percent.

25. The sample characterizing instrument of claim 18, said excitation light source further comprising a fourth monochromator.