RANTES homolog antibody

• US 6,238,666 B1
• Filed: 09/14/1999
• Issued: 05/29/2001
• Est. Priority Date: 04/17/1996
• Status: Expired due to Fees

• Current Assignee: Incyte Corporation
• Original Assignee: Incyte Corporation
• Inventors: Bandman, Olga, Hawkins, Phillip R., Murry, Lynn E.
• Primary Examiner(s): Gambel, Phillip
• Assistant Examiner(s): ROARK, JESSICA HOPE
• Application Number:
  US09/395,918
• Time in Patent Office: 623 Days
• Field of Search: 424/130.1, 424/133.1, 424/135.1, 424/137.1, 424/139.1, 424/141.1, 424/145.1, 435/4, 435/7.1, 530/387.1, 530/387.3, 530/387.9, 530/388.1, 530/388.23, 530/389.1, 530/389.2, 530/412, 530/413
• US Class Current: 424/145.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 11/06 Antiasthmatics
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/00 Drugs for immunological or allergic disorders
• CPC Class Codes: A61P 37/08 Antiallergic agents antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14
• CPC Class Codes: C07K 14/523 Beta-chemokines, e.g. RANTES, I-309/TCA-3, MIP-1alpha, MIP-1beta/ACT-2/LD78/SCIF, MCP-1/MCAF, MCP-2, MCP-3, LDCF-1, LDCF-2
• CPC Class Codes: C12N 2799/026 where the vector is derived from a baculovirus
• CPC Class Codes: Y10S 930/14 Lymphokine; related peptides

Abstract

The present invention provides a polynucleotide (PTEC) isolated from a prostate cDNA library which identifies and encodes a human rantes homolog (PTEC). The invention provides for genetically engineered expression vectors and host cells comprising the nucleic acid sequence encoding PTEC. The invention also provides for the therapeutic use of purified PTEC in the treatment of immune deficiency diseases, and for the therapeutic use of antisense molecules, antibodies, antagonists or inhibitors in the treatment of conditions or diseases associated with the expression of PTEC. The invention also describes diagnostic assays which utilize diagnostic compositions comprising the polynucleotide, or fragments thereof, or antibodies which specifically bind to the polypeptide.

Claims

1. An antibody specific for human RANTES homolog having the amino acid sequence of SEQ ID NO:

2.

2. The antibody of claim 1, wherein the antibody is:

(a) a chimeric antibody;

(b) a single chain antibody;

or

(c) a Fab fragment;

or

(d) a F(ab′

)₂ fragment.

3. The antibody of claim 2, wherein the antibody is produced by screening a Fab expression library.

4. The antibody of claim 2, wherein the antibody is produced by screening a recombinant immunoglobulin library.

5. A composition comprising the antibody of claim 1 in conjunction with a pharmaceutical carrier.

6. A method for using an antibody to detect expression of the polypeptide of SEQ ID NO:

2 in a biological sample comprising the steps of;

a) combining the biological sample with the antibody of claim 1, under conditions for the antibody to bind the polypeptide and form an antibody;

polypeptide complex; and

b) detecting the complex, wherein the presence of the complex indicates the expression of the polypeptide in the biological sample.

7. A method of using an antibody to purify a polypeptide of SEQ ID NO:

2 from media, the method comprising;

a) attaching the antibody of claim 1 to an immunoaffinity column;

b) passing the media over the column under conditions to allow binding between the antibody and the polypeptide; and

c) separating the antibody from the polypeptide, thereby obtaining purified polypeptide.