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DNA sequences by mass spectrometry

  • US 6,238,871 B1
  • Filed: 05/08/2000
  • Issued: 05/29/2001
  • Est. Priority Date: 01/07/1993
  • Status: Expired due to Term
First Claim
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1. A method for determining the sequence of a nucleic acid comprising the steps of:

  • a) generating at least two base-specifically terminated fragments from a nucleic acid to be sequenced that comprises nucleotides with a modification at a base, a sugar or a phosphate of a nucleotide, wherein the modification improves the separation or resolution of the fragments when analyzed compared to unmodified fragments;

    b) desorbing and ionizing the base-specifically terminated fragments with a single laser source;

    c) simultaneously determining the molecular weight value of each of the desorbed and ionized base-specifically terminated fragments by mass spectrometry, and d) determining the sequence of the nucleic acid by aligning the base-specifically terminated fragments according to molecular weight.

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