Methods and materials for the diagnosis and treatment of sporadic basal cell carcinoma

• US 6,238,876 B1
• Filed: 06/22/1998
• Issued: 05/29/2001
• Est. Priority Date: 06/20/1997
• Status: Expired due to Term

• Current Assignee: New York University
• Original Assignee: New York University
• Inventors: Altaba, Ariel Ruiz i
• Primary Examiner(s): Bui, Phuong T.
• Application Number:
  US09/102,491
• Time in Patent Office: 1,072 Days
• Field of Search: 435/7.23, 435/7.2, 435/7.1, 435/70.3, 435/371, 436/64, 530/350, 530/828, 536/23.5
• US Class Current: 435/7.23
• CPC Class Codes: G01N 33/5082 Supracellular entities, e.g. tissue, organisms
• CPC Class Codes: G01N 33/5743 of skin, e.g. melanoma
• CPC Class Codes: Y10S 530/828 Cancer

Abstract

Methods for detection of the onset or presence of sporadic basal cell carcinoma in an animal by measuring for elevated levels of ectopic expression of Gli1 in the animal'"'"'s epidermal tissue sample suspected of harboring sporadic basal cell carcinoma.

Claims

1. A method for detection of the onset or presence of sporadic basal cell carcinoma in an animal, said method comprising

providing a skin sample from said animal suspected of harboring sporadic basal cell carcinoma,

measuring the level of Gli1 in said sample, and

detecting the presence or onset of sporadic basal cell carcinoma based on said measurement of Gli1, where in elevated levels of Gli1 above normal indicate said presence or onset of sporadic basal cell carcinoma.

2. A method for detection of the onset or presence of sporadic basal cell carcinoma in an animal, said method comprising

providing a skin sample from said animal suspected of harboring sporadic basal cell carcinoma,

culturing said sample with either a frog embryo or a test cell colony, said culturing being for a time sufficient for development of skin tumors on said frog embryo or tumor formation in said test colony if said sample contains elevated levels of ectopically expressed Gli1 above normal, and

examining said frog embryo for skin tumors or said test cell colony for tumor formation, wherein development of frog embryo skin tumors or test cell colony tumor formation is indicative of the presence or onset of sporadic basal cell carcinoma.