Culture and transportation of bovine embryos

• US 6,238,920 B1
• Filed: 02/17/1993
• Issued: 05/29/2001
• Est. Priority Date: 01/19/1993
• Status: Expired due to Fees

• Current Assignee: National Agriculture and Food Research Organization (Government of Japan)
• Original Assignee: Director of Livestock Improvement Association of Japan Incorporated, Director of National Food Research Institute
• Inventors: Takahashi, Masashi, Nagai, Takashi, Okano, Akira, Kuwayama, Masashige, Hirako, Hitomi, Kadokawa, Hiroya, Hamano, Seizo, Kariya, Takayoshi
• Primary Examiner(s): Weber, Jon P.
• Application Number:
  US08/018,546
• Time in Patent Office: 3,023 Days
• Field of Search: 435/240.1, 435/240.2, 435/240.25, 435/240.3, 435/1, 435/2, 435/374, 435/325, 435/383
• US Class Current: 435/374
• CPC Class Codes: A01N 1/02 Preservation of living parts
• CPC Class Codes: A01N 1/0226 Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients

Abstract

A method of culturing bovine embryos by culturing said bovine embryos in a medium containing thiol compound, and also a method of transporting bovine embryos by transporting said bovine embryos in a medium containing thiol compound are disclosed.

According to the present invention, it is possible to culture bovine embryos up to blastocyst stage efficiently in the medium containing low molecular thiol compound without in vivo culture or coculture using feeder cells. Also when low molecular thiol compound is added to the transportation medium for bovine embryos, almost 100% embryos are still viable after long distant transportation.

Claims

1. A method of transporting bovine embryos developed to the blastocyst stage in a culture medium and which have not been frozen which comprises transferring the embryos from the culture medium to a transportation medium and transporting said bovine embryos in the transportation medium, the transportation medium comprising a thiol compound selected from the group consisting of β

-mercaptoethanol, β

-mercaptoethylamine, glutathione, dithiothreitol and α

-thioglycerol, while maintained in non-frozen condition, the transporting being from a culturing laboratory to a location for carrying out embryo transfer.

2. The method of claim 1, wherein said bovine embryos are transported while maintained at 37°

C.

3. The method of claim 2, wherein the thiol compound is present at a concentration of 10-150 μ

M in the transportation medium.

4. The method of claim 3, wherein the thiol compound is β

-mercaptoethanol.

5. The method of claim 1, wherein the thiol compound is β

-mercaptoethanol.