Device for high throughout sample processing, analysis and collection, and methods of use thereof

• US 6,240,790 B1
• Filed: 06/18/1999
• Issued: 06/05/2001
• Est. Priority Date: 11/09/1998
• Status: Expired due to Term

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Brennen, Reid A., Swedberg, Sally A
• Primary Examiner(s): RAEVIS, ROBERT R
• Application Number:
  US09/336,521
• Time in Patent Office: 718 Days
• Field of Search: 738/63, 738/632.1, 738/632.3, 738/632.5, 738/633.1, 738/633.3, 738/648.1, 738/648.3, 738/648.5, 422/68.1, 422/69, 422/70, 210/601, 210/602, 210/451, 210/452
• US Class Current: 73/863.21
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0874 Three dimensional network
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces

Abstract

A microanalysis device having a plurality of sample processing compartments is described for use in liquid phase analysis. A microanalysis device system, comprising a plurality of interconnected microanalysis devices. The device is formed by microfabrication of microstructures in novel support substrates. The invention herein can be used for the analysis of small and/or macromolecular and/or other solutes in the liquid phase.

Claims

1. A device for use in microanalysis, comprising a substrate having:

(a) first and second substantially planar opposing surfaces; and

(b) a plurality of microfabricated parallel sample processing compartments each comprising

(i) a sample treatment component,

(ii) an inlet port in fluid communication with the sample treatment component, and

(iii) an outlet port in fluid communication with the sample treatment component.

2. The device of claim 1, wherein the inlet and outlet ports are in the first and second opposing surfaces, respectively, of the substrate.

3. The microanalysis device of claim 1, wherein the inlet port, the outlet port, or both the inlet and outlet ports are in a lateral surface of the substrate.

4. The device of claim 1, wherein the sample processing compartment further comprises a sample flow component.

5. The device of claim 1, wherein at least one sample processing compartment further comprises a sample flow component in serial arrangement with the sample treatment component.

6. The device of claim 5, wherein the serial arrangement of the sample flow component and the sample treatment component is alternating.

7. The device of claim 1, wherein the inlet port or the outlet port enable inter-microanalysis device fluid communication.

8. The device of claim 7, wherein the inlet port and the outlet port enable inter-microanalysis device fluid communication.

9. The device of claim 1, wherein each of the sample treatment components performs the same function.

10. The device of claim 9, wherein each of the sample treatment components comprise the same element.

11. The device of claim 9, wherein each of the sample treatment components comprises a different element.

12. The microanalysis device of claim 1, wherein each of the sample treatment components performs a different function.

13. The device of claim 1, wherein the outlet port is in fluid communication with a sample detection means.

14. The microanalysis device of claim 13, wherein the outlet port is in fluid communication with an off-device sample detection means.

15. The device of claim 13, wherein the outlet port is in fluid communication with an on-device sample detection means.

16. The microanalysis device of claim 1, wherein the outlet port is in fluid communication with a sample separation chamber.

17. The microanalysis device of claim 16, wherein the outlet port is in fluid communication with an off-device sample separation chamber.

18. The microanalysis device of claim 16, wherein the outlet port is in fluid communication with an on-device sample separation chamber.