Spill-resistant microtitre trays and method of making

• US 6,241,949 B1
• Filed: 08/17/1999
• Issued: 06/05/2001
• Est. Priority Date: 08/17/1999
• Status: Expired due to Term

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Spectrumedix Corporation
• Inventors: Kane, Thomas E.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Handy, Dwayne K.
• Application Number:
  US09/376,076
• Time in Patent Office: 658 Days
• Field of Search: 422/99-104, 435/287, 435/293, 435/300, 435/301, 222/143, 222/330
• US Class Current: 422/553
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates

Abstract

Microtitre trays and their spill-resistant open lids prevent cross contamination of samples and also accommodate fragile and flexible probes of automated biological sample analysis systems. The open-lids can be in the form of sleeves located on the top sides of each well of microtitre trays. In one version, one microtitre tray with its bottom sections severed is stacked on top of another microtitre tray to provide the open-lids. A cutting tool is also provided to sever the bottom sections of each well from microtitre trays. The cutting tool includes a lever, a blade and a clamping device.

Claims

1. A method of manufacturing a spill-resistant microtitre tray structure, comprising:

providing a modified microtitre tray which includes regularly spaced apart wells, each well having an open top and an open bottom located at opposite ends of the well;

providing an unmodified microtitre tray which includes wells having a same spacing as those of the modified microtitre tray, each well having an open top and a closed bottom located at opposite ends of the respective well, a shape of the wells belonging to the unmodified tray being identical to a shape of the wells belonging to the modified tray except for the open bottoms of the wells on the modified tray; and

inserting at least a plurality of wells of the modified microtitre tray into a plurality of wells of the unmodified microtitre tray, to thereby form a stacked arrangement in which the plurality of wells of the modified microtitre tray serve as spill-resistant lids for the wells of the unmodified microtitre tray into which the plurality of wells are inserted.

2. The method according to claim 1 wherein the modified microtitre tray is provided by molding a microtitre tray having wells with open tops and open bottoms.

3. The method according to claim 1 wherein the modified microtitre tray is provided by severing bottoms of wells of a microtitre tray having wells with closed bottoms.

4. The method according to claim 3 wherein the step of severing the bottom sections further includes the step of:

simultaneously cutting the bottom section of each well.

5. The method according to claim 1, wherein the modified tray and the unmodified tray are identical in shape, except for the open bottoms of the wells on the modified tray.

6. A spill-resistant microtitre tray structure, comprising:

a modified microtitre tray including regularly spaced apart wells, each well having an open top and an open bottom located at opposite ends of the well; and

an unmodified microtitre tray which includes wells having a same spacing as those of the modified microtitre tray, each well having an open top and a closed bottom located at opposite ends of the well,

wherein at least a plurality of the wells of the modified microtitre tray are inserted into a plurality of wells of the unmodified microtitre tray to thereby form a stacked arrangement in which the plurality of wells of the modified microtitre tray serve as spill-resistant lids for the plurality of wells of the unmodified microtitre tray, and

wherein a shape of the wells belonging to the unmodified tray is identical to a shape of the wells belonging to the modified tray except for the open bottoms of the wells on the modified tray.

7. The structure according to claim 6 wherein the open tops and open bottoms of the plurality of wells of the modified microtitre tray are sufficiently large to receive a plurality of probes.

8. The structure according to claim 6 wherein the plurality of wells of the modified microtitre tray are made from a semi-rigid plastic material such that the plurality of wells of the modified microtitre tray are deformed when inserted into the plurality of wells of the unmodified microtitre tray,

to thereby form a friction fit between the modified microtitre tray and the unmodified microtitre tray.

9. The structure according to claim 6 wherein the stacked arrangement includes a contact region where the unmodified and modified trays touch each other, and

wherein the contact region includes an adhesive.

10. The structure according to claim 6, wherein each of the modified and unmodified microtitre trays includes a two dimensional array of wells.

11. The spill-resistant microtitre tray structure according to claim 6, wherein the modified tray and the unmodified tray are identical in shape, except for the open bottoms of the wells on the modified tray.