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In-line complete hyperspectral fluorescent imaging of nucleic acid molecules

  • US 6,245,507 B1
  • Filed: 08/18/1998
  • Issued: 06/12/2001
  • Est. Priority Date: 08/18/1998
  • Status: Expired due to Term
First Claim
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1. A method for hyperspectral imaging a fluorescently labeled nucleotide analog wherein said method comprises the steps:

  • (a) emitting a transmission beam from a light source for hyperspectral imaging;

    (b) expanding said transmission beam by passing said transmission beam through an expansion lens that expands said transmission beam for microarray detection;

    (c) focusing said expanded transmission beam into a focus line for microarray detection by passing said expanded transmission beam through a focusing lens;

    (d) contacting said fluorescently labeled nucleotide analog with said focused transmission beam, wherein said contact between said focused transmission line and said fluorescently labeled nucleotide analog excites said fluorescently labeled nucleotide analog to emit a fluorescent emission;

    (e) collecting said fluorescent emission with a collection lens;

    (f) projecting said collected fluorescent emission into an imaging spectrometer for hyperspectral imaging; and

    (g) detecting said projected fluorescent emission using a detector.

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