Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
First Claim
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1. A method for recovering DNA, RNA or proteins from a formalin-fixed paraffin-embedded biological tissue sample comprising:
- deparaffinizing the sample;
heating the sample in a chaotropic solution comprising an effective concentration of a guanidinium compound to a temperature in the range of about 60 to about 100°
C. for a time period of about 30 to about 120 minutes; and
recovering said RNA, DNA or protein from said chaotropic solution.
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Abstract
Methods are disclosed for rapid, reliable and simple isolation of RNA from formalin-fixed paraffin-embedded tissue samples. RNA purified in this manner can be used to monitor gene expression levels. The tissue sample can be a tumor or other pathological tissue.
159 Citations
32 Claims
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1. A method for recovering DNA, RNA or proteins from a formalin-fixed paraffin-embedded biological tissue sample comprising:
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deparaffinizing the sample;
heating the sample in a chaotropic solution comprising an effective concentration of a guanidinium compound to a temperature in the range of about 60 to about 100°
C. for a time period of about 30 to about 120 minutes; and
recovering said RNA, DNA or protein from said chaotropic solution. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A method for isolating RNA from a formalin-fixed paraffin-embedded biological tissue sample comprising:
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deparaffinizing the sample;
heating the sample in a chaotropic solution comprising an effective concentration of a guanidinium compound to a temperature in the range of about 75 to about 100°
C. for a time period of about 30 to about 60 minutes; and
recovering said RNA from said chaotropic solution. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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Specification