×

Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens

  • US 6,248,535 B1
  • Filed: 12/20/1999
  • Issued: 06/19/2001
  • Est. Priority Date: 12/20/1999
  • Status: Expired due to Term
First Claim
Patent Images

1. A method for recovering DNA, RNA or proteins from a formalin-fixed paraffin-embedded biological tissue sample comprising:

  • deparaffinizing the sample;

    heating the sample in a chaotropic solution comprising an effective concentration of a guanidinium compound to a temperature in the range of about 60 to about 100°

    C. for a time period of about 30 to about 120 minutes; and

    recovering said RNA, DNA or protein from said chaotropic solution.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×