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System and methods for nucleic acid sequencing of single molecules by polymerase synthesis

  • US 6,255,083 B1
  • Filed: 12/13/1999
  • Issued: 07/03/2001
  • Est. Priority Date: 12/14/1998
  • Status: Expired due to Term
First Claim
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1. A method of genotyping or sequencing a target nucleic acid comprising the steps of;

  • i. immobilizing onto a solid support a complex comprising a target nucleic acid, a primer nucleic acid which complements a region of the target nucleic acid, and at least one nucleic acid polymerase;

    ii. contacting the immobilized complex with at least one type of labeled nucleotide triphosphate (NTP), wherein each type of NTP is differently labeled with a detectable label which is released when said NTP is incorporated, and iii. detecting the incorporation of a labeled NTP into a single molecule of the primer by detecting a unique label released from the labeled NTP, to genotype or to sequence the target nucleic acid.

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