Artificial antibodies to corticosteroids prepared by molecular imprinting

• US 6,255,461 B1
• Filed: 04/05/1996
• Issued: 07/03/2001
• Est. Priority Date: 04/05/1996
• Status: Expired due to Fees

• Current Assignee: Klaus Mosbach, Olof Ramstrom
• Original Assignee: Klaus Mosbach, Olof Ramstrom
• Inventors: Mosbach, Klaus, Ramstrom, Olof
• Primary Examiner(s): CEPERLEY, MARY
• Application Number:
  US08/628,381
• Time in Patent Office: 1,915 Days
• Field of Search: 530/389.2, 264/330, 264/331.18
• US Class Current: 530/389.2
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: B01J 20/26 Synthetic macromolecular compounds
• CPC Class Codes: B01J 20/261 obtained by reactions only involving carbon to carbon unsaturated bonds macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds per se C08F
• CPC Class Codes: B01J 20/268 Polymers created by use of a template, e.g. molecularly imprinted polymers
• CPC Class Codes: B01J 20/3057 Use of a templating or imprinting material molecularly imprinted polymers B01J20/268; filling pores of a substrate or matrix followed by the removal of the substrate or matrix
• CPC Class Codes: C07K 16/26 against hormones ; against hormone releasing or inhibiting factors
• CPC Class Codes: G01N 33/531 Production of immunochemical test materials
• CPC Class Codes: G01N 33/743 Steroid hormones

Abstract

The invention relates to artificial antibodies that are prepared by molecular imprinting, where methacrylic acid, ethylene glycol dimethacrylate and a print molecule are combined to form an artificial antibody having spatially positioned binding sites dictated by the corticosteroid print molecule, and the antibodies can be used in separation and analytical procedures.

Claims

1. An artificial antibody, comprising:

non-covalently molecular imprinted copolymers having pre-determined, spatially positioned specific binding sites for a corticosteroid, wherein said copolymers are prepared by copolymerizing methacrylic acid with ethylene glycol dimethacrylate in the presence of a porogenic solvent and in the presence of a corticosteroid print molecule, wherein said porogenic solvent is tetrahydrofuran or acetone, and wherein said print molecule is cortisol or corticosterone.

2. The antibody according to claim 1, wherein said porogenic solvent is tetrahydrofuran.

3. The antibody according to claim 1, wherein said porogenic solvent is acetone.

4. The antibody according to claim 1, wherein said print molecule is cortisol.

5. The antibody according to claim 1, wherein said print molecule is corticosterone.

6. A method for producing an artificial polymeric antibody by non-covalent molecular imprinting, comprising:

i) combining methacrylic acid as a first monomer with corticosteroid print molecules in the presence of a porogenic solvent, wherein said print molecules are cortisol or corticosterone, and said porogenic solvent is tetrahydrofuran or acetone;

ii) adding ethylene glycol dimethacrylate as a crosslinking monomer to the methacrylic acid and print molecules of said step i);

iii) polymerizing said monomers to form a polymeric antibody by non-covalent molecular imprinting; and

iv) removing the print molecules after step iii) to form an artificial polymeric antibody having predetermined spatially positioned, specific binding sites for corticosteroids.

7. The method according to claim 6, wherein said porogenic solvent is acetone.

8. The method according to claim 6, wherein said porogenic solvent is tetrahydrofuran.

9. The method according to claim 6, wherein said print molecules are cortisol.

10. The method according to claim 6, wherein said print molecules are corticosterone.