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Chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation

  • US 6,255,475 B1
  • Filed: 09/16/1997
  • Issued: 07/03/2001
  • Est. Priority Date: 01/31/1995
  • Status: Expired due to Term
First Claim
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1. A compound of the structure I:

  • Or a salt thereof, whereinB is a nucleobase, X and Z independently are oxygen or sulphur, Y is hydrogen or a protected or unprotected hydroxy, R1 is hydrocarbyl, which optionally is substituted with a functional group selected from the group consisting of tertiary amino, nitro, cyano and halogen, R2 is hydrogen hydrocarbyl, which optionally is substituted with a functional group selected from the group consisting of tertiary amino, nitro, cyano and halogen, A is an electron withdrawing or electron donating group capable of moderating the acetyl stability of the compound I, L1 and L2 are hydrocarbon linkers, which may be the same or different, L2, when present, being either (I) connected to L1 via the group A, or (ii) directly connected to L1, the group A then being connected to one of linkers L1 and L2, F is a dye label, Q is a coupling group for F, and l, m and n independently are 0 or 1, with the proviso that l is 1 when m is 1, and l is 1 and m is 1 when n is 1.

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