Microbial polyunsaturated fatty acid containing oil from pasteurised biomass

• US 6,255,505 B1
• Filed: 03/19/1997
• Issued: 07/03/2001
• Est. Priority Date: 03/28/1996
• Status: Expired due to Term

• Current Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Original Assignee: Gist-Brocades N.V. (DSM-Firmenich AG)
• Inventors: Visser, Johannes Martinus Jacobus, Bijl, Hendrik Louis, Schaap, Albert, Wolf, Johannes Hendrik
• Primary Examiner(s): CARR, DEBORAH D
• Application Number:
  US08/821,026
• Time in Patent Office: 1,567 Days
• Field of Search: 554/8, 554/20, 554/227, 435/254.1, 435/257.1, 435/132, 435/134, 435/171
• US Class Current: 554/227
• CPC Class Codes: A23D 9/00 Other edible oils or fats, e.g. shortenings, cooking oils
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23L 31/00 Edible extracts or preparations of fungi; Preparation or treatment thereof
• CPC Class Codes: A23L 33/11 Plant sterols or derivatives thereof, e.g. phytosterols
• CPC Class Codes: A23L 33/12 Fatty acids or derivatives thereof
• CPC Class Codes: A23L 33/15 Vitamins
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A23V 2250/1868 Docosahexaenoic acid
• CPC Class Codes: A23V 2250/1882 Polyunsaturated fatty acids
• CPC Class Codes: A23V 2250/706 Vitamin B12
• CPC Class Codes: A61K 2800/85 Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
• CPC Class Codes: A61K 8/375 the alcohol moiety containing more than one hydroxy group
• CPC Class Codes: A61K 8/67 Vitamins
• CPC Class Codes: A61K 8/92 Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
• CPC Class Codes: A61K 8/9728 Fungi, e.g. yeasts
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: C07C 51/47 by solid-liquid treatment; by chemisorption
• CPC Class Codes: C07C 57/03 Monocarboxylic acids
• CPC Class Codes: C07C 57/12 Straight chain carboxylic acids containing eighteen carbon atoms
• CPC Class Codes: C11B 1/02 Pretreatment
• CPC Class Codes: C11B 1/10 : by extracting
• CPC Class Codes: C11B 1/104 : using super critical gases or vapours
• CPC Class Codes: C12N 1/005 : after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
• CPC Class Codes: C12N 1/14 : Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: C12P 17/06 : containing a six-membered hetero ring, e.g. fluorescein
• CPC Class Codes: C12P 19/42 : Cobalamins, i.e. vitamin B12, LLD factor
• CPC Class Codes: C12P 23/00 : Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes containing heterorings C12P17/00
• CPC Class Codes: C12P 7/6427 : Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
• CPC Class Codes: C12P 7/6432 : Eicosapentaenoic acids [EPA]
• CPC Class Codes: C12P 7/6434 : Docosahexenoic acids [DHA]
• CPC Class Codes: C12P 7/6463 : obtained from glyceride producing microorganisms, e.g. single cell oil
• CPC Class Codes: C12P 7/6472 : containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Abstract

The present invention discloses a microbial polyunsaturated fatty acid(PUFA)-containing oil with a high triglyceride content and a high oxidative stability. In addition, a method is described for the recovery of such oil from a microbial biomass derived from a pasteurised fermentation broth, wherein the microbial biomass is subjected to extrusion to form granular particles, dried and the oil then extracted from the dried granules using an appropriate solvent.

Claims

1. A microbial oil, comprising at least one polyunsaturated fatty acid (PUFA), which has a triglyceride content greater than 93%.

2. An oil according to claim 1 having a Rancimat induction time of a ≧

5 hours at 80°

C.

3. An oil according to claim 1 wherein the PUFA is a C18, C20 or C22 ω

-3 or a C18, C20 or C22 ω

-6 polyunsaturated fatty acid.

4. An oil according to claim 3 wherein the PUFA is a C20 or C22 ω

-3 or a C20 ω

-6 polyunsaturated fatty acid.

5. An oil according to claim 1 wherein the PUFA is arachidonic acid (ARA), eicosapentaenoic acid (EPA) and/or docosahexaenoic acid (DHA).

6. An oil according to claim 1 wherein the PUFA is only ARA.

7. An oil according to claim 1 wherein the PUFA is produced by a fungus.

8. An oil according to claim 1 wherein the fungus is of the genus Mortierella.

9. An oil according to claim 1 wherein the PUFA is produced by an alga.

10. An oil according to claim 8 wherein the alga is of the genus Crypthecodinium.

11. A food, feed, cosmetic or nutritional supplement for humans or animals comprising an oil according to any one of claims 1 to 10.

12. The food composition according to claim 11 which is an infant formula.

13. The food composition according to claim 11 which is milk, a health drink or bread.