Biosensor

• US 6,258,254 B1
• Filed: 07/27/1998
• Issued: 07/10/2001
• Est. Priority Date: 07/28/1997
• Status: Expired due to Term

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Miyamoto, Yoshiko, Ikeda, Shin, Yoshioka, Toshihiko, Nankai, Shiro
• Primary Examiner(s): WARDEN, ROBERT J
• Assistant Examiner(s): Aldag, Andrew
• Application Number:
  US09/122,628
• Time in Patent Office: 1,079 Days
• Field of Search: 204/403, 205/777.5, 205/778, 435/287.9, 435/4, 435/817
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/004 mediator-assisted

Abstract

A method using a biosensor is disclosed which facilitates high accuracy quantitation of a substrate by best eliminating adverse effects of red blood cell existing in blood together with the substrate. The biosensor comprises an electrode system including a working electrode and a counter electrode formed on an electrically insulating base plate, and a reaction layer containing at least an oxidoreductase and an electron acceptor, wherein the electron acceptor is a sodium salt.

Claims

1. A method for measuring a component in whole blood comprising:

applying said whole blood to an electrode system, said electrode system including a working electrode and a counter electrode formed on an electrically insulating base plate,

applying a voltage to said working electrode, and

allowing said component in said whole blood to react with a reaction layer, said reaction layer containing at least an oxidoreductase and an electron acceptor, wherein said electron acceptor is a sodium salt, and wherein said sodium salt facilitates measurement of said component in said whole blood without adversely affecting erythrocytes in said whole blood.

2. A method in accordance with claim 1, wherein said sodium salt is sodium ferricyanide.

3. A method in accordance with claim 1, wherein said reaction layer further contains a hydrophilic polymer.